Adam M Mccoy

US Patent:

20120329664, Dec 27, 2012

Filed:

Mar 19, 2012

Appl. No.:

13/424304

Inventors:

Serge Saxonov - Oakland CA, US
Simant Dube - Pleasanton CA, US
Benjamin J. Hindson - Livermore CA, US
Adam M. McCoy - Davis CA, US

Assignee:

BIO-RAD LABORATORIES, INC. - Hercules CA

International Classification:

C40B 30/04
C40B 40/06
G01N 21/64

US Classification:

506 9, 435 611, 506 16

Abstract:

System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

US Patent:

20070031869, Feb 8, 2007

Filed:

Jul 12, 2006

Appl. No.:

11/486329

Inventors:

Adam McCoy - Milpitas CA, US
Stephen Palumbi - Pacific Grove CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Template specific inhibition during PCR (TSI-PCR) allows specific inhibition of particular templates without disrupting the amplification of other templates that share amplification primer binding sites. TSI-PCR is achieved by ligation of stop oligos comprising a 3′ modification that prevents extension by DNA polymerases. Stop oligos hybridize to a region of the targeted amplicon downstream of the amplification primer. When the stop oligos are perfectly complementary to the target template, they are ligated onto the extending strand during the extension phase of the amplification cycle. This effectively truncates the extending strand at the site where the stop oligo binds and blocks further extension. The truncated products are not full-length and cannot be extended, and therefore do not serve as additional templates during sunsequent rounds of amplification. This results in substantial inhibition over multiple amplification cycles, but only for templates that match the stop oligos.

US Patent:

20180258476, Sep 13, 2018

Filed:

Nov 8, 2016

Appl. No.:

15/758065

Inventors:

- Carlsbad CA, US
John D. CURRY - Concord CA, US
Robert O'CALLAHAN - Spring TX, US
Adam MCCOY - Davis CA, US
Daniel FITZPATRICK - Santa Clara CA, US
Philip H. DICKINSON - Cupertino CA, US
Anthony C. SCHWEITZER - Redwood City CA, US

International Classification:

C12Q 1/6848
C12Q 1/6876
C12Q 1/6827
C12Q 1/6853

Abstract:

Disclosed are compositions, methods and kits for determining the presence, absence, amount, copy number, or other characteristics of one or more polynucleotide sequences in two or more samples and use thereof in genotyping, evaluation of copy number variation, expression analysis, determination of splice variants and fusion genes, and other genetic analyses.