Skills:
Experienced in the aseptic culture and maintenance of mammalian cell lines, experienced in the culture of rat and human primary cells, Chromosome conformation capture (3C) and Chromosome conformation capture carbon-copy (5C), Chromatin immunoprecipitations, microarray technology, molecular cloning, bacterial transformations, transfections, primer design and PCR analysis, RNA isolation and purification from cells and tissue, RT-PCR, Real-time PCR, lactate quantification, ATP quantification, Apoptotic assays including Hoescht staining and caspase-3 cleavage, Cell cycle analysis, Flow cytometry, Mitochondrial isolation and complex function, Mitochondrial membrane potential analysis, western blotting, mouse genotyping, extensive expertise in yeast genetics