Alan E Blanchard

US Patent:

6384210, May 7, 2002

Filed:

Mar 13, 2000

Appl. No.:

09/381487

Inventors:

Alan P. Blanchard - Kenmore WA

Assignee:

University of Washington - Seattle WA

International Classification:

C07H 2100

US Classification:

536 253, 536 231, 536 243, 422129, 422131, 347 1, 347 7, 347 40, 347 85, 347107

Abstract:

The present invention provides a method of biopolymer, esp. oligonucleotide, synthesis. The method consists of coupling a first nucleotide to a second nucleotide in a high surface tension solvent. The invention also provides microdroplets of a soln. comprising a solvent having a b. p. of 150 degree. C. or above, a surface tension of 30 dynes/cm or above, and a viscosity of 0. 015 g/(cm)(sec), e. g. , propylene carbonate. Such microdroplets are useful for the synthesis of chem. species, particularly biopolymers such as oligonucleotides and peptides, as well as arrays of chem. species. An automated system for oligonucleotide synthesis is described, which comprises delivery of microdroplets by inkjet technol. and computer control of the process. The high surface tension solvent used is selected for compatibility with the inkjet technol.

US Patent:

6419883, Jul 16, 2002

Filed:

Jan 16, 1998

Appl. No.:

09/008120

Inventors:

Alan P. Blanchard - Seattle WA

Assignee:

University of Washington - Seattle WA

International Classification:

B32B 3500

US Classification:

422134, 4221022, 422111, 4221291, 422131, 422136, 42214911

Abstract:

The present invention relates to microdroplets of a solution comprising a solvent having a boiling point of 150Â C. or above, a surface tension of 30 dynes/cm or above, and a viscosity of 0. 015 g/(cm)(sec). Such microdroplets are useful for the synthesis of chemical species, particularly biopolymers such as oligonucleotides and peptides, as well as arrays of chemical species. Preferably, the solvent has the formula (I): wherein A=O or S; X=O, S or N (C -C alkyl) Y=O, S, N(C -C alkyl) or CH ; and R=C -C straight or branched chain alkyl.

US Patent:

20010018512, Aug 30, 2001

Filed:

Mar 26, 2001

Appl. No.:

09/817491

Inventors:

Alan Blanchard - Seattle WA, US

Assignee:

University of Washington

International Classification:

C07H021/02
C07H021/04
C07H021/00
C08F002/00
B32B005/02
B32B027/12
B32B027/04

US Classification:

536/025300, 536/023100, 536/024300, 422/131000

Abstract:

The present invention relates to microdroplets of a solution comprising a solvent having a boiling point of 150 C. or above, a surface tension of 30 dynes/cm or above, and a viscosity of 0.015 g/(cm)(sec). Such microdroplets are useful for the synthesis of chemical species, particularly biopolymers such as oligonucleotides and peptides, as well as arrays of chemical species. Preferably, the solvent has the formula (I): wherein A=O or S; X, O, S or N(C-Calkyl); Y=O, S, N(C-Calkyl) or CH; and R=C-Cstraight or branched chain alkyl.

US Patent:

58761870, Mar 2, 1999

Filed:

Mar 9, 1995

Appl. No.:

8/401546

Inventors:

Fred K. Forster - Seattle WA
Ronald L. Bardell - Seattle WA
Alan P. Blanchard - Seattle WA
Martin A. Afromowitz - Mercer Is. WA
Nigel R. Sharma - Bothel WA

Assignee:

University of Washington - Seattle WA

International Classification:

F04B 1700

US Classification:

417322

Abstract:

Micropumps fabricated by micromachining techniques and employing fixed or no-moving-parts valves. As one aspect of the invention, a laser-assisted chemical etching technique is employed for providing smooth-walled, curved configuration necessary to obtain the desired flow characteristics of the valves that are used in conjunction with the micropump.

US Patent:

60281898, Feb 22, 2000

Filed:

Mar 20, 1997

Appl. No.:

8/821156

Inventors:

Alan P. Blanchard - Seattle WA

Assignee:

University of Washington - Seattle WA

International Classification:

C07H 2100
C07H 2102
C07H 2104

US Classification:

536 253

Abstract:

The invention provides methods for assembling arrays of oligonucleotides on a solid support, wherein the arrays can optionally be removed from the support after assembly. The invention also provides a solvent suitable for automated assembly of arrays of oligonucleotides.