Albert A George

US Patent:

58560890, Jan 5, 1999

Filed:

Jul 22, 1994

Appl. No.:

8/279315

Inventors:

Mary Ge Wang - Rockville MD
Albert Louis George - Gaithersburg MD
Elizabeth Sophia Light - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934
C07H 2104
C07H 2102

US Classification:

435 6

Abstract:

The present invention is directed to in situ hybridization methods using nucleic acid probes for single copy sequences for detecting chromosomal structural abnormalities in fixed tissue obtained from a patient suspected of having a chromosomal structural abnormality.

US Patent:

57285268, Mar 17, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/472239

Inventors:

Albert L. George - Gaithersburg MD
Satish K. Bhatnagar - Gaithersburg MD
Irina Nazarenko - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

A method for analyzing a target nucleotide sequence which exists in a first state or a different second state which makes the method particularly useful for determining point mutations. The method uses a first polynucleotide which is immobilized on a solid support and which is at least partially complementary to a first segment of the target nucleotide sequence. By means of a series of steps, a product of the first polynucleotide and a further polynucleotide that contains a detectable label can be obtained. When the state to be analyzed occurs in a rare population, amplification can be conducted so that substantially only amplification of the target nucleotide sequence in one of the states is attained. The method can be used to analyze multiple target sequences simultaneously. A kit which can be used in the method is also set forth.

US Patent:

54515025, Sep 19, 1995

Filed:

Aug 31, 1993

Appl. No.:

8/114997

Inventors:

Albert L. George - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

US Patent:

51027844, Apr 7, 1992

Filed:

May 4, 1990

Appl. No.:

7/519146

Inventors:

Albert L. George - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12N 912
G01N 33566
G01N 3348

US Classification:

435 6

Abstract:

The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. A second oligonucleotide is present in the reaction that is homologous to the 3 prime end of the probe molecule and also conains 5 prime base or bases that will reconstitute the restriction enzyme site on the target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

US Patent:

55938400, Jan 14, 1997

Filed:

Jun 5, 1995

Appl. No.:

8/461823

Inventors:

Satish K. Bhatnagar - Gaithersburg MD
Albert L. George - Gaithersburg MD
Irina Nazarenko - Gaithersburg MD

Assignee:

Oncor, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12Q 170
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

A process for amplifying nucleic acid sequences from a DNA or RNA template which may be purified, or may exist in a mixture of nucleic acids. The resulting nucleic acid sequences may be exact copies of the template, or may be modified. The process has advantages over prior art amplification processes in that it increases the fidelity of copying a specific nucleic acid sequence, and it allows one to more efficiently detect a particular point mutation in a single assay.