Alexander M Rowe

US Patent:

20180223275, Aug 9, 2018

Filed:

Nov 29, 2017

Appl. No.:

15/826671

Inventors:

- Pittsburgh PA, US
Adriana Turqueti Neves - Olching, DE
Eduardo Schittler Neves - Olching, DE
Constantinos George Panousis - Pittsburgh PA, US
Alexander McIntyre Rowe - Pittsburgh PA, US

International Classification:

C12N 15/10
C12N 5/0783
C07K 14/725
C12P 19/34

Abstract:

This document provides methods and materials involved in cloning functional TCRs from single T cells. For example, methods and materials for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, kits for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, methods for making such kits, collections of nucleic acid primers designed to amplify the entire coding sequence of both variable regions for each expressed V segment for functional αβ or γδ TCRs of a particular mammalian species, methods for using such collections of nucleic acid primers to clone functional TCRs from single T cells, and kits containing such collections of nucleic acid primers to clone functional TCRs from single T cells are provided.