- Pleasanton CA, US Tim CRAIG - Mountain View CA, US Alexander YANG - Mountain View CA, US Liv JENSEN - Mountain View CA, US Charlotte YANG - Mountain View CA, US Corissa HARRIS - Mountain View CA, US Matthew DIPIETRO - Gilroy CA, US Dhruti DALAL - Sunnyvale CA, US
The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.
- Santa Clara CA, US - Mannheim, DE Alexander H. Yang - Campbell CA, US Liv E. Jensen - Palo Alto CA, US Marshall Porter - Santa Clara CA, US Charlotte Yang - San Jose CA, US Corissa Harris - Santa Clara CA, US Matt Dipetro - Gilroy CA, US
Assignee:
Genia Technologies, Inc. - Santa Clara CA
International Classification:
C12Q 1/6869 C12N 9/12
Abstract:
A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.
- Santa Clara CA, US Tim CRAIG - Mountain View CA, US Alexander YANG - Mountain View CA, US Liv JENSEN - Mountain View CA, US Charlotte YANG - Mountain View CA, US Corissa HARRIS - Mountain View CA, US
Assignee:
GENIA TECHNOLOGIES, INC. - Santa Clara CA
International Classification:
C07K 14/245 C12N 9/12 C12Q 1/6874
Abstract:
The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.
Name / Title
Company / Classification
Phones & Addresses
Alexander Yang General Manager
Susan's Dance Studio Dance Studio/School/Hall
2146 Ringwood Ave, San Jose, CA 95131 4084351889
Alexander Yang President
FLASH TECH, INC
1300 John Reed Ct STE A, Hacienda Heights, CA 91745 1300 John Reed Ct, Whittier, CA 91745
Doctors Hospital Of Manteca Anesthesiology 1205 E North St, Manteca, CA 95336 2092398391 (phone), 2092398396 (fax)
Education:
Medical School University of Kansas School of Medicine Graduated: 1993
Languages:
Chinese English Portuguese Spanish
Description:
Dr. Yang graduated from the University of Kansas School of Medicine in 1993. He works in Manteca, CA and specializes in Anesthesiology. Dr. Yang is affiliated with Doctors Hospital Of Manteca.