Ali M Mohamed

US Patent:

20220280564, Sep 8, 2022

Filed:

Feb 23, 2021

Appl. No.:

17/281095

Inventors:

- Houston TX, US
Mamta KALRA - Sugar Land TX, US
Ali MOHAMED - Sugar Land TX, US
Steffen WALTER - Houston TX, US
Yannick BULLIARD - Thousand Oaks TX, US

International Classification:

A61K 35/17
C07K 14/725
C12N 5/0783

Abstract:

An in vitro method of expanding γδ T cells includes isolating γδ T cells from a blood sample of a human subject, activating the isolated γδ T cells in the presence of an aminobisphosphonate and/or a feeder cell and at least one cytokine, expanding the activated γδ T cells, and optionally restimulating the expanded γδ T cells.

US Patent:

20210285011, Sep 16, 2021

Filed:

Mar 10, 2021

Appl. No.:

17/197927

Inventors:

- Houston TX, US
Mamta KALRA - Sugar Land TX, US
Yannick BULLIARD - Thousand Oaks CA, US
Steffen WALTER - Houston TX, US
Ali MOHAMED - Sugar Land TX, US

International Classification:

C12N 15/86
C12N 15/10
C07K 14/725
C12N 15/113

Abstract:

The present invention provides a mutated woodchuck post-transcriptional regulatory element (WPRE). In particular, the present invention relates to a mutated WPRE sequence that can efficiently express nucleotides of interest in a retroviral vector system. The present invention also relates to methods of delivering and expressing nucleotides of interest to a target cell.

US Patent:

20210030803, Feb 4, 2021

Filed:

Oct 2, 2020

Appl. No.:

17/062331

Inventors:

- Houston TX, US
Zoe Coughlin - Richmond TX, US
Amir Alpert - Houston TX, US
Steffen Walter - Houston TX, US
Ali Mohamed - Sugar Land TX, US
Agathe Bourgogne - Houston TX, US

International Classification:

A61K 35/17
C12N 5/0783
C07K 16/28
C12N 15/86
A61P 31/00
A61P 35/00

Abstract:

The disclosure relates to methods of manufacturing T cells for adoptive immunotherapy. The disclosure further provides for methods of genetically transducing T cells, methods of using T cells, and T cell populations thereof. In an aspect, the disclosure provides for methods of thawing frozen peripheral blood mononuclear cells (PBMC), resting the thawed PBMC, activating the T cell in the cultured PBMC with an anti-CD3 antibody and an anti-CD28 antibody immobilized on a solid phase, transducing the activated T cell with a viral vector, expanding the transduced T cell, and obtaining expanded T cells.

US Patent:

20210002610, Jan 7, 2021

Filed:

Jun 4, 2020

Appl. No.:

16/893246

Inventors:

- Houston TX, US
- Tübingen, DE
Anastasiya SMITH - Houston TX, US
Claudia WAGNER - Tuebingen, DE
Ali MOHAMED - Sugar Land TX, US

International Classification:

C12N 5/0783
A61K 31/5383
A61K 31/437

Abstract:

Described herein are methods for preparing T cells, including isolating CD8+ T cells from a blood sample obtained from a patient or a donor, culturing the isolated CD8+ T cells in the presence of at least one cytokine, contacting the cultured CD8+ T cells with a multimer containing a target peptide in a complex with an MHC molecule and with at least one binding agent that binds to a T cell surface molecule, in which the multimer is labelled with a first detectable agent and the binding agent is labelled with a second detectable agent, sorting the contacted CD8+ T cells to collect the sorted CD8+ T cells that are detected positive for the first and the second detectable agents, and expanding the collected CD8+ T cells.

US Patent:

20190247433, Aug 15, 2019

Filed:

Feb 8, 2019

Appl. No.:

16/271393

Inventors:

- Houston TX, US
Zoe COUGHLIN - Houston TX, US
Amir ALPERT - Houston TX, US
Steffen WALTER - Houston TX, US
Ali MOHAMED - Houston TX, US
Agathe BOURGOGNE - Houston TX, US

International Classification:

A61K 35/17
C12N 5/0783
C07K 16/28
C12N 15/86
A61P 35/00
A61P 31/00

Abstract:

The disclosure relates to methods of manufacturing T cells for adoptive immunotherapy. The disclosure further provides for methods of genetically transducing T cells, methods of using T cells, and T cell populations thereof. In an aspect, the disclosure provides for methods of thawing frozen peripheral blood mononuclear cells (PBMC), resting the thawed PBMC, activating the T cell in the cultured PBMC with an anti-CD3 antibody and an anti-CD28 antibody immobilized on a solid phase, transducing the activated T cell with a viral vector, expanding the transduced T cell, and obtaining expanded T cells.

US Patent:

20190216852, Jul 18, 2019

Filed:

Jan 17, 2019

Appl. No.:

16/250679

Inventors:

- Houston TX, US
Agathe BOURGOGNE - Houston TX, US
Ali MOHAMED - Houston TX, US
Steffen WALTER - Houston TX, US

International Classification:

A61K 35/17
C12N 7/00
C12N 15/85
A61P 35/00

Abstract:

The present disclosure relates to T cells transduced with a viral vector at a volumetric concentration for immunotherapy and methods thereof. In another aspect, the present disclosure relates to the assessment of optimal lentiviral vector concentrations for transducing T cells. The present disclosure further provides for T cell populations produced by methods described herein.