Wilson Moya - Concord MA, US Alison Dupont - Sandown NH, US
Assignee:
EMD MILLIPORE CORPORATION - Billerica MA
International Classification:
C07K 1/36
US Classification:
5303873, 5303871, 5303881, 530415
Abstract:
Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
Use Of Small Molecules In Methods For Purification Of Biomolecules
Jad Jaber - Sudbury MA, US Wilson Moya - Concord MA, US Ajish Potty - Woburn MA, US Alison Dupont - Sandown NH, US Matthew T. Stone - Cambridge MA, US Mikhail Kozlov - Lexington MA, US
Assignee:
EMD Millipore Corporation - Billerica MA
International Classification:
C07K 16/00
US Classification:
5303881
Abstract:
The present invention relates to novel and improved methods for the purification of biomolecules. In particular, the present invention relates to methods of protein purification which employ small molecules, which include at least one non-polar group and at least one cationic group or which include at least one non-polar group and at least one anionic group.
Wilson Moya - Concord MA, US Alison Dupont - Sandown NH, US
Assignee:
MILLIPORE CORPORATION - Billerica MA
International Classification:
C07K 16/00 C12M 1/00 C07K 1/14
US Classification:
5303873, 4352891, 530415, 5303891
Abstract:
Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
Tangential Flow Filtration Device For Perfusion Applications
- Burlington MA, US Alison Dupont - Lyndeborough NH, US
International Classification:
B01D 63/10 C12M 1/00
Abstract:
Filter elements for perfusion systems and methods are provided. A filter element sheet includes a microporous membrane having a mean pore size of at least about 0.65 μm and a feed spacer comprising woven fibers and having an open area of at least about 35%. The filter element sheet can be arranged within a filter element, for example, in a spiral-wound format or in a cassette format. A perfusion system includes at least one filter element and a pump configured to control flow of a liquid feed through the at least one filter element.
- Burlington MA, US Alison Dupont - Lyndeborough NH, US
International Classification:
B01D 61/18 C12M 1/00
Abstract:
The disclosure herein relates to a TFF assembly including a baffle comprising a microporous filter within a bioreactor. The baffle may include a collection receptacle to capture a target product or waste materials within the baffle after passing through the microporous filter.