Alison Mui Tsan from 122 Saint Jean Ct, Danville, CA 94526, age 47, Phone: 5624819360

Preferential Amplification Of Mrna Over Dna Using Chemically Modified Primers
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US Patent:

8614071, Dec 24, 2013
Filed:

Dec 8, 2010
Appl. No.:

12/962890
Inventors:

Stephen Will - Cham, CH
Lori Steiner - Alameda CA, US
Alison Tsan - Castro Valley CA, US
Nicolas Newton - Oakland CA, US
Assignee:

Roche Molecular Systems, Inc. - Pleasanton CA
International Classification:

C12P 19/34
C07H 21/00
B01L 3/00
US Classification:

435 911, 536 231, 422430
Abstract:

The present invention relates to a method, oligonucleotides, reaction mixtures and kits for the selective amplification of a messenger RNA target comprising an exon-exon junction, using an oligonucleotide that comprises at least one nucleotide modified at the exocyclic amino group.

Allele-Specific Amplification
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US Patent:

20100099110, Apr 22, 2010
Filed:

Oct 20, 2009
Appl. No.:

12/582068
Inventors:

Stephen G. Will - Rotkreuz, CH
Alison Tsan - Castro Valley CA, US
Nicolas Newton - Oakland CA, US
Assignee:

ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00
US Classification:

435 6, 435 912, 536 231
Abstract:

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3′-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3′-terminal nucleotide.

Allele-Specific Amplification Of Nucleic Acids
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US Patent:

20110311968, Dec 22, 2011
Filed:

Dec 8, 2010
Appl. No.:

12/962861
Inventors:

Stephen Will - Cham, CH
Alison Tsan - Castro Valley CA, US
Nicolas Newton - Oakland CA, US
Assignee:

ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:

C12P 19/34
C07H 21/00
C12N 9/12
C12Q 1/68
US Classification:

435 611, 435 912, 536 243, 435194
Abstract:

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, having an internally-placed selective nucleotide complementary to only one variant of the target sequence wherein the allele-specific oligonucleotide is extended by a nucleic acid polymerase predominantly or exclusively when hybridized to the variant of the target sequence for which it has said complementary selective nucleotide.

Methods And Compositions For Detecting Mutation In The Human Epidermal Growth Factor Receptor Gene
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US Patent:

20120164641, Jun 28, 2012
Filed:

Dec 13, 2011
Appl. No.:

13/324705
Inventors:

Keith Bauer - San Rafael CA, US
Nancy Schoenbrunner - Moraga CA, US
Alison Tsan - Castro Valley CA, US
Assignee:

ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:

C12Q 1/68
C07H 21/00
US Classification:

435 611, 536 231
Abstract:

The invention comprises reagents and methods for detecting cancer-causing mutations in the human EGFR gene. Further, a method of detecting the mutations and a method of treatment are disclosed.

Use Of G-Clamp For Improved Allele-Specificpcr
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US Patent:

20130078630, Mar 28, 2013
Filed:

Sep 21, 2012
Appl. No.:

13/624077
Inventors:

Nancy Schoenbrunner - Moraga CA, US
Alison Tsan - Castro Valley CA, US
Assignee:

ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:

C12Q 1/68
C12N 9/12
C07H 21/04
C12P 19/34
US Classification:

435 611, 435 912, 435194, 536 2431
Abstract:

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating at least one “G-clamp” nucleotide.

Novel Complex Mutations In The Epidermal Growth Factor Receptor Kinase Domain
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US Patent:

20130121996, May 16, 2013
Filed:

Oct 30, 2012
Appl. No.:

13/664333
Inventors:

Alison Tsan - Castro Valley CA, US
Assignee:

ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:

C12Q 1/68
A61P 35/00
A61K 31/517
A61K 31/5377
C07H 21/04
A61K 39/395
US Classification:

4241331, 435 611, 536 2431, 4241421, 5142664, 5142345
Abstract:

Six new mutations were found in exon 19 of the EGFR gene, the exon that is often mutated in tumors. The invention comprises methods of detecting the mutations, methods of prognosis and methods of predicting response to treatment based on the presence of absence of the mutations.

Detection Of Target Nucleic Acid By Solid-Phase Molography
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US Patent:

20220298555, Sep 22, 2022
Filed:

Dec 19, 2019
Appl. No.:

17/633359
Inventors:

- Pleasanton CA, US
Igor Kozlov - Danville CA, US
Nikolaus-Peter Stengele - Pleiskirchen, DE
Alison Tsan - Danville CA, US
Volker Gatterdam - Nuesttal, DE
International Classification:

C12Q 1/6825
C12Q 1/6853
Abstract:

The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography.

Methods For Performing Multiplexed Real-Time Pcr
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US Patent:

20210269857, Sep 2, 2021
Filed:

May 7, 2021
Appl. No.:

17/314470
Inventors:

- Pleasanton CA, US
Amar Gupta - Danville CA, US
Randall Saiki - Alameda CA, US
Alison Tsan - Danville CA, US
International Classification:

C12Q 1/686
C12Q 1/6823
C12Q 1/6811
C12Q 1/6883
C12Q 1/6804
C12Q 1/6818
C12Q 1/6837
Abstract:

The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

Alison Mui Tsan