Amy L Hamilton

US Patent:

53488873, Sep 20, 1994

Filed:

Nov 13, 1992

Appl. No.:

7/976301

Inventors:

Thomas F. Bumol - Carmel IN
Robert A. Gadski - Indianapolis IN
Amy E. Hamilton - Indianapolis IN
Joann Strnad - Yardley PA

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 1512
C12N 1570

US Classification:

4353201

Abstract:

The present invention comprises novel recombinant DNA compounds which encode the. about. 40,000 dalton adenocarcinoma antigen recognized by monoclonal antibody KS 1/4. Eukaryotic and prokaryotic expression vectors have been constructed that comprise novel KSA-encoding DNA and drive expression of KSA when transformed into an appropriate host cell. The novel expression vectors can be used to produce KSA derivatives, such as non-glycosylated KSA, and to produce KSA precursors, such as nascent KSA, and to produce subfragments of KSA. The recombinant-produced KSA is useful for the diagnosis, prognosis and treatment of disease states including adenocarcinomas of the lung, prostate, breast, ovary and colon/rectum; and for the creation of novel antibodies for treatment or diagnosis of the above.