Amy Anjanette Rogers

US Patent:

7785786, Aug 31, 2010

Filed:

Jan 23, 2006

Appl. No.:

11/337805

Inventors:

Maurice Exner - Mission Viejo CA, US
Amy Rogers - Dana Point CA, US

Assignee:

Quest Diagnostics Investments Incorporated - Wilmington DE

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 2431, 536 2432, 536 2433

Abstract:

Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.

US Patent:

8039216, Oct 18, 2011

Filed:

Jul 28, 2010

Appl. No.:

12/845465

Inventors:

Maurice Exner - Mission Viejo CA, US
Amy Rogers - Dana Point CA, US

Assignee:

Quest Diagnostics Investments Incorporated - Wilmington DE

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 612, 435 912, 536 24312, 536 2432, 536 2433

Abstract:

Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.