Andrew R Reeves

US Patent:

7638306, Dec 29, 2009

Filed:

Aug 8, 2003

Appl. No.:

10/637159

Inventors:

J. Mark Weber - Chicago IL, US
Andrew R. Reeves - Chicago IL, US
Igor A. Brikun - Forest Park IL, US
William Henry Cernota - Chicago IL, US

Assignee:

Fermalogic, Inc. - Chicago IL

International Classification:

C12P 19/62
C12N 9/00
C12N 9/10
C12N 1/20

US Classification:

435 76, 435183, 435193, 4352523

Abstract:

A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

US Patent:

8039239, Oct 18, 2011

Filed:

Dec 16, 2008

Appl. No.:

12/336278

Inventors:

Andrew Reeves - Chicago IL, US

Assignee:

Coskata, Inc. - Warrenville IL

International Classification:

C12P 7/06
C12P 7/16
C12N 9/04
C12N 1/20
C01B 3/00

US Classification:

435161, 435160, 435190, 4352527, 252373

Abstract:

Recombinant acetogenic Clostridia are engineered to modulate production of aliphatic C-Calcohols and aliphatic C-Corganic acids from synthetic gases. One aspect of the invention provides a method of producing an aliphatic C-Calcohol using an acetogenic micro-organism having at least one genetic modification that reduces or eliminates C-Ccarboxylic acid production by the modified organism. Another aspect of the invention provides a method of producing an aliphatic C-Calcohol using an acetogenic micro-organism having one or more genetic modifications that cause increased enzyme activity of carbon monoxide dehydrogenase, aldehyde ferredoxin oxidoreductase, NADPH-dependent alcohol dehydrogenase, or alcohol dehydrogenase. Yet another aspect of the invention provides a method of producing aliphatic C2-C6 alcohols using acetogenic micro-organisms that have been genetically modified to increase C-Caliphatic alcohol formation and decrease the production of the corresponding C-Caliphatic organic acid.

US Patent:

8551746, Oct 8, 2013

Filed:

Sep 21, 2011

Appl. No.:

13/239305

Inventors:

Andrew Reeves - Chicago IL, US
Rathin Datta - Chicago IL, US

Assignee:

Coskata, Inc. - Warrenville IL

International Classification:

C12P 7/52
C12P 7/06

US Classification:

435140, 435161, 435157, 435141

Abstract:

A method of operating a fermentation zone for the production of ethanol from syngas uses a crotonate derivative to prevent or reverse the effects of butyrigen contamination. The crotonate compound works in continuous fermentation processes to reduce or eliminate contamination from butyrate and butanol in the syngas derived ethanol product.

US Patent:

8628943, Jan 14, 2014

Filed:

Jun 9, 2010

Appl. No.:

12/802560

Inventors:

Andrew Reeves - Chicago IL, US
Rathin Datta - Chicago IL, US

Assignee:

Coskata, Inc. - Warrenville IL

International Classification:

C12P 7/06
C12N 9/00
C12N 1/00
C12N 1/20
C12N 15/00
C07H 21/04

US Classification:

435161, 435183, 435243, 4352523, 4353201, 536 232

Abstract:

Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established. Key genes of interest for metabolic catalyzing activity in clostridial species include a three-gene operon coding for CODH activity, a two-gene operon coding for PTA-ACK, and a novel acetyl coenzyme A reductase. The promoter regions of the two operons and the acetyl coA reductase are manipulated to increase ethanol production.

US Patent:

20070122885, May 31, 2007

Filed:

Aug 22, 2006

Appl. No.:

11/466364

Inventors:

Andrew Reeves - Chicago IL, US
J. Weber - Chicago IL, US
Igor Brikun - Forest Park IL, US

Assignee:

FERMALOGIC, INC. - Chicago IL

International Classification:

C12P 19/62
C12N 9/10
C12N 15/74
C12N 1/21

US Classification:

435076000, 435252350, 435471000, 435193000

Abstract:

A process of increasing the cellular production of secondary metabolites, such as antibiotics, is provided. The process is particularly useful for increasing antibiotic production by bacterial cells, especially erythromycin. The process includes the step of increasing the activity of methylmalonyl-CoA mutase.

US Patent:

20070128702, Jun 7, 2007

Filed:

Dec 1, 2006

Appl. No.:

11/566155

Inventors:

J. Weber - Chicago IL, US
Andrew Reeves - Chicago IL, US
Igor Brikun - Forest Park IL, US

Assignee:

FERMALOGIC, INC. - Chicago IL

International Classification:

C12P 19/62
C12N 15/74
C12N 1/21

US Classification:

435076000, 435252300, 435252350, 435471000

Abstract:

Consumable biotech strain improvement products are presented, as well as methods of preparation and using them. The technology is based on reversible, single-crossover insertion vectors, such as plasmids or phage. Because the single crossover event is reversible in the absence of drug selection, the products cannot be maintained in a useful form without knowledge of the drug selection agent. Consumable strain improvement products can be constructed with 1st generation reverse engineering protections, having at least 25%-75% of the effectiveness of the equivalent traditional (permanent) strain improvement product under laboratory condition.

US Patent:

20090311677, Dec 17, 2009

Filed:

Jun 13, 2008

Appl. No.:

12/157978

Inventors:

Andrew Reeves - Chicago IL, US
Richard E. Tobey - St. Charles IL, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 6, 536 2433

Abstract:

A method for determining the presence of a contaminating organism in a fermentation process includes initiating the fermentation process using a syngas and obtaining a first aliquot from the fermentation process. The method further includes subjecting said first aliquot to a polymerase chain reaction using at least one oligonucleotide primer capable of hybridizing to a target sequence of a genomic nucleic acid from a suspected contaminating organism, producing a first amplified product based on the polymerase chain reaction, separating the first amplified product based on size, and determining the presence of the suspected contaminating organism based on the first separated amplified product.

US Patent:

20100047899, Feb 25, 2010

Filed:

May 19, 2009

Appl. No.:

12/468456

Inventors:

J. Mark Weber - Chicago IL, US
Andrew R. Reeves - Chicago IL, US
Igor A. Brikun - Forest Park IL, US
William Henry Cernota - Chicago IL, US

International Classification:

C12N 1/20

US Classification:

4352521

Abstract:

A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.