Angela B Reynolds

US Patent:

7595387, Sep 29, 2009

Filed:

Dec 22, 2004

Appl. No.:

11/019831

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Peter S. Linsley - Seattle WA, US

Assignee:

Dharmacon, Inc. - Lafayette CO
Merck & Co., Inc. - Rahway NJ

International Classification:

C07H 21/04

US Classification:

536 245, 536 2431, 435 6, 435325, 435375, 514 44

Abstract:

Methods and compositions for performing RNA interference with decreased off-target effects are provided The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2′-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.

US Patent:

7834171, Nov 16, 2010

Filed:

Sep 19, 2007

Appl. No.:

11/857732

Inventors:

Devin Leake - Denver CO, US
Angela R Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US

Assignee:

Dharmacon, Inc. - Lafayette CO

International Classification:

C07H 21/04
A61K 48/00

US Classification:

536 245, 536 2431, 536 241, 514 44, 435 6, 435325, 435375

Abstract:

Methods and compositions for performing RNA interference with decreased off-target effects are provided. The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2′-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.

US Patent:

8546349, Oct 1, 2013

Filed:

Jul 26, 2011

Appl. No.:

13/190797

Inventors:

Anja Smith - Thornton CO, US
Angela Reynolds - Littleton CO, US
Jon E. Chatterton - Fort Worth TX, US
Xinyu Zhang - Southlake TX, US

Assignee:

Thermo Fisher Scientific Biosciences Inc. - Lafayette CO
Alcon Research, Ltd. - Fort Worth TX

International Classification:

C12N 15/11

US Classification:

514 44A

Abstract:

Vascular endothelial growth factor A (VEGFA) is a chemical signal produced by cells that stimulates the growth of new blood vessels, and overexpression of VEGFA can lead to undesirable physiological conditions. Through the identification of new siRNA and modifications that improve the silencing ability of these siRNA in vivo, therapeutic compositions and methods have been invented to address the problems associated with this overexpression.

US Patent:

20040198640, Oct 7, 2004

Filed:

Apr 2, 2003

Appl. No.:

10/406908

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Stephen Scaringe - Lafayette CO, US

Assignee:

Dharmacon, Inc. - Lafayette CO

International Classification:

A61K048/00

US Classification:

514/008000, 514/044000

Abstract:

Methods and compositions for performing RNA interference comprising a wide variety of stabilized polynucleotides suitable for use in serum-containing media and for in vivo applications, such as therapeutic applications, are provided. These polynucleotides permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of one or more modifications including orthoesters, terminal conjugates, modified linkages and 2′modified nucleotides.

US Patent:

20040224405, Nov 11, 2004

Filed:

May 6, 2003

Appl. No.:

10/431027

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US

Assignee:

Dharmacon Inc. - Lafayette CO

International Classification:

A61K048/00
C12N005/02

US Classification:

435/375000, 514/044000

Abstract:

The present invention is directed to methods and compositions for performing gene silencing in mammalian cells by targeting a region of a non-protein coding target nucleic acid sequence with at least one siRNA molecule comprising a duplex region of between 19 and 30 base pairs.

US Patent:

20040266707, Dec 30, 2004

Filed:

Jul 1, 2003

Appl. No.:

10/613077

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Denver CO, US
Anastasia Khvorova - Denver CO, US
William Marshall - Denver CO, US

International Classification:

A61K048/00
C07H015/24
C07H021/02
C07J017/00

US Classification:

514/044000, 536/006100, 536/023100

Abstract:

Methods and compositions for performing RNA interference comprising a wide variety of stabilized polynucleotides suitable for use in serum-containing media and for in vivo applications, such as therapeutic applications, are provided. These polynucleotides permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of one or more modifications including orthoesters, terminal conjugates, modified linkages and 2′modified nucleotides.

US Patent:

20050176045, Aug 11, 2005

Filed:

Jan 25, 2005

Appl. No.:

11/043637

Inventors:

Yuriy Fedorov - Superior CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US

Assignee:

Dharmacon, Inc. - Lafayette CO

International Classification:

C12Q001/68
C12N015/85

US Classification:

435006000, 435455000

Abstract:

A method of identifying SNP specific siRNA is provided. The method comprises comparing the silencing effect of: (i) at least two SNP containing siRNA in cells that contain a SNP target sequence; (ii) said at least two SNP containing siRNA in cells that contain a wild type target sequence; (iii) at least two non-SNP containing siRNA in cells that contain a SNP target sequence; and (iv) said at least two non-SNP containing siRNA in cells that contain a wild type target sequence. Through the method, SNP specific siRNA can be selected for a diverse set of genes, including the Kras gene.

US Patent:

20070167384, Jul 19, 2007

Filed:

Apr 1, 2004

Appl. No.:

10/551350

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Yuriy Fedorov - Superior CO, US
Kimberly Nichols - Louisville CO, US

Assignee:

DHARMACON, INC. - Lafayette CO

International Classification:

A61K 48/00
C07J 17/00
C07H 21/02

US Classification:

514044000, 514026000, 536006100, 536023100

Abstract:

p-00010000