Rachel T. Noble - Beaufort NC, US Angelia D. Blackwood - Newport NC, US
Assignee:
The University of North Carolina at Chapel Hill - Chapel Hill NC
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NO:1-4, the novel oligonucleotide probe sequences set forth in SEQ ID NO:5-8, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly and spp. in a sample.
Enterococcus And Fecal Bacteroides For Rapid Water Quality Assessment
Angelia Denene Blackwood - Newport NC, US Rachel T. Noble - Beaufort NC, US
International Classification:
G01N 21/64
US Classification:
435 611
Abstract:
The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NOS: 1-4, 7 and 8, the novel oligonucleotide probe sequences set forth in SEQ ID NOS:5, 6, and 9, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly spp. and fecal spp., or a specimen processing control, in a sample.
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