Angelia Denene Blackwood

US Patent:

20080233572, Sep 25, 2008

Filed:

Sep 21, 2007

Appl. No.:

11/859032

Inventors:

Rachel T. Noble - Beaufort NC, US
Angelia D. Blackwood - Newport NC, US

Assignee:

The University of North Carolina at Chapel Hill - Chapel Hill NC

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NO:1-4, the novel oligonucleotide probe sequences set forth in SEQ ID NO:5-8, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly and spp. in a sample.

US Patent:

20120190025, Jul 26, 2012

Filed:

Jul 22, 2010

Appl. No.:

13/387219

Inventors:

Angelia Denene Blackwood - Newport NC, US
Rachel T. Noble - Beaufort NC, US

International Classification:

G01N 21/64

US Classification:

435 611

Abstract:

The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NOS: 1-4, 7 and 8, the novel oligonucleotide probe sequences set forth in SEQ ID NOS:5, 6, and 9, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly spp. and fecal spp., or a specimen processing control, in a sample.