Skills:
Molecular Biology: 454 sequencing, In-Fusion, Polymerase Chain Reaction (PCR) troubleshooting, BAC DNA purification and analysis using restriction enzyme digest, HAMILTON robot operation and protocol development, PFGE of BAC DNA, BAC Homologous Recombination, Southern blot, AmpureBead DNA cleanup, MacVector software, Sequencher software, all basic molecular techniques. Protein Biochemistry: MembraneMax: cell-free membrane protein expression, protein expression in: E. coli, CHO cells, and cell free, protein column purification, protein quantitation, Western blot, immunoprecipitation. Cell Biology: ES cell culture, adult stem cell culture, adult stem cell isolation from human blood, mammalian cell culture, transfection, organelle purification using percoll gradient, sucrose gradient purifications, Scanning and Transmission Electron Microscopy training.