Annmarie B Tuttle

US Patent:

56591241, Aug 19, 1997

Filed:

Jan 3, 1995

Appl. No.:

8/368773

Inventors:

Lyle D. Crossland - Chapel Hill NC
Annmarie Tuttle - Garner NC
Jeffrey I. Stein - Chapel Hill NC

Assignee:

Novartis Corporation - Tarrytown NY

International Classification:

A01H 500
C12N 1582
C12N 1529
C12N 1533

US Classification:

800205

Abstract:

The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

US Patent:

54098238, Apr 25, 1995

Filed:

Sep 24, 1992

Appl. No.:

7/950348

Inventors:

Lyle D. Crossland - Chapel Hill NC
Annmarie Tuttle - Garner NC
Jeffrey I. Stein - Chapel Hill NC

Assignee:

Ciba-Geigy Corporation - Ardsley NY

International Classification:

C12N 1582
C12N 1579
A01H 500

US Classification:

4351723

Abstract:

The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

US Patent:

58245421, Oct 20, 1998

Filed:

May 23, 1995

Appl. No.:

8/449514

Inventors:

Lyle D. Crossland - Chapel Hill NC
Annmarie Tuttle - Garner NC
Jeffrey I. Stein - Chapel Hill NC

Assignee:

Novartis Finance Corporation - New York NY

International Classification:

C12N 1582
C12N 1529
C12N 1531
A01H 102

US Classification:

4353201

Abstract:

The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed. The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.

US Patent:

54770025, Dec 19, 1995

Filed:

Mar 7, 1994

Appl. No.:

8/207904

Inventors:

Annmarie B. Tuttle - Garner NC
Lyle D. Crossland - Chapel Hill NC

Assignee:

Ciba-Geigy Corporation - Ardsley NY

International Classification:

A01H 500
C12N 1511
C12N 1582

US Classification:

800205

Abstract:

cDNA sequences are disclosed which are expressed specifically in the anther of a plant. Genomic DNA sequences corresponding to the cDNA clones are obtained using the cDNA clones as hybridization probes. Recombinant, or chimeric, DNA sequences are constructed in which the promoter sequence from anther-specific genomic clones are operatively linked to a DNA sequence coding for a desired polypeptide. Transgenic plants are made in which the chimeric DNA sequences are expressed in the anther of the transgenic plant. In a preferred embodiment, the coding DNA sequence expresses a polypeptide which will disrupt formation of viable pollen, resulting in a male-sterile plant.