Anthony D Person

US Patent:

20130189741, Jul 25, 2013

Filed:

Dec 31, 2012

Appl. No.:

13/731802

Inventors:

Anthony Person - Madison WI, US
Cynthia Chin - Madison WI, US
Jerome Jendrisak - Middleton WI, US
Gary Dahl - Madison WI, US

Assignee:

Cellscript, Inc. - Madison WI

International Classification:

C12N 5/071

US Classification:

435 912, 435377, 435196

Abstract:

The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.

US Patent:

20110143436, Jun 16, 2011

Filed:

Dec 7, 2010

Appl. No.:

12/962498

Inventors:

Gary Dahl - Madison WI, US
Anthony Person - Madison WI, US
Judith Meis - Fitchburg WI, US
Jerome Jendrisak - Madison WI, US

International Classification:

C12N 5/00
C07H 21/02

US Classification:

435377, 536 235

Abstract:

The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.

US Patent:

20110143397, Jun 16, 2011

Filed:

Dec 7, 2010

Appl. No.:

12/962468

Inventors:

Katalin Kariko - Rydal PA, US
Drew Weissman - Wynnewood PA, US
Gary Dahl - Madison WI, US
Anthony Person - Madison WI, US
Judith Meis - Fitchburg WI, US
Jerome Jendrisak - Madison WI, US

International Classification:

C12N 5/071
C12P 21/00

US Classification:

435 703, 435377, 435366

Abstract:

The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

US Patent:

20220257795, Aug 18, 2022

Filed:

Aug 31, 2021

Appl. No.:

17/463007

Inventors:

- Madison WI, US
Anthony Person - Madison WI, US
Cynthia Chin - Madison WI, US
Jerome Jendrisak - Middleton WI, US
Gary Dahl - Madison WI, US

International Classification:

A61K 48/00
C12P 21/02
A61K 38/00
A61K 31/7105
C12P 19/34
C12N 5/074
C12N 15/10
C12N 5/0793
A61K 33/06
C12N 5/077
C12N 15/87
C12P 19/38

Abstract:

The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

US Patent:

20210079346, Mar 18, 2021

Filed:

Sep 28, 2020

Appl. No.:

17/034999

Inventors:

- Madison WI, US
Anthony D. Person - Madison WI, US
Gary A. Dahl - Madison WI, US

International Classification:

C12N 5/078
A61K 38/16
C07K 14/705
C07K 14/715
C07K 14/005
A61K 31/7105
A61K 38/17
A61K 38/21
A61K 31/711
C12N 5/077
C12N 5/074

Abstract:

The present invention provides methods, kits, and compositions for reducing an innate immune system response in a human or animal cell, tissue or organism. One embodiment comprises: introducing an Agent mRNA comprising in vitro-synthesized mRNA encoding one or more proteins that affect the induction, activity or response of an innate immune response pathway; whereby, the innate immune response in the cell, tissue or organism is reduced compared to the innate immune response in the absence of the Agent mRNA. Other embodiments are methods, compositions and kits for using an Agent mRNA for treating a disease or medical condition in a human or animal that exhibits symptoms of an elevated innate immune system, or for reducing an innate immune response that is induced in a human or animal cell, tissue or organism by a Foreign Substance that is administered to the cell, tissue or organism.

US Patent:

20210024895, Jan 28, 2021

Filed:

Mar 23, 2020

Appl. No.:

16/827098

Inventors:

- Philadelphia PA, US
Drew Weissman - Wynnewood PA, US
Gary Dahl - Madison WI, US
Anthony Person - Madison WI, US
Judith Meis - Fitchburg WI, US
Jerome Jendrisak - Madison WI, US

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12N 5/074
C12N 15/87
C12N 15/85
A61K 38/17
A61K 38/18
A61K 38/44
A61K 38/46
A61K 38/50
A61K 48/00
C07K 14/47
C07K 14/505
C12N 9/02
C12N 9/22
C12N 9/78
C12N 15/117

Abstract:

The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

US Patent:

20190321490, Oct 24, 2019

Filed:

Dec 19, 2018

Appl. No.:

16/225489

Inventors:

- Madison WI, US
Anthony Person - Madison WI, US
Cynthia Chin - Madison WI, US
Jerome Jendrisak - Middleton WI, US
Gary Dahl - Madison WI, US

International Classification:

A61K 48/00
C12P 19/34
C12N 5/074
C12N 15/10
C12N 5/0793
A61K 33/06
C12P 21/02
A61K 38/00
C12P 19/38
C12N 15/87
A61K 31/7105
C12N 5/077

Abstract:

The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

US Patent:

20180312806, Nov 1, 2018

Filed:

Nov 28, 2017

Appl. No.:

15/823869

Inventors:

- Madison WI, US
Anthony D. Person - Madison WI, US
Gary A. Dahl - Madison WI, US

International Classification:

C12N 5/078
A61K 38/16
C07K 14/005
C12N 5/074
C12N 5/077
A61K 31/711
A61K 38/21
A61K 38/17
C07K 14/705
A61K 31/7105
C07K 14/715

Abstract:

The present invention provides methods, kits, and compositions for reducing an innate immune system response in a human or animal cell, tissue or organism. One embodiment comprises: introducing an Agent mRNA comprising in vitro-synthesized mRNA encoding one or more proteins that affect the induction, activity or response of an innate immune response pathway; whereby, the innate immune response in the cell, tissue or organism is reduced compared to the innate immune response in the absence of the Agent mRNA. Other embodiments are methods, compositions and kits for using an Agent mRNA for treating a disease or medical condition in a human or animal that exhibits symptoms of an elevated innate immune system, or for reducing an innate immune response that is induced in a human or animal cell, tissue or organism by a Foreign Substance that is administered to the cell, tissue or organism.