Katayoun Zand - Irvine CA, US Ted Pham - Westminster CA, US Antonio Davila - Philadelphia PA, US Douglas Wallace - Swarthmore PA, US
International Classification:
G01N 33/58
US Classification:
436 63, 422502, 422505, 422 8208
Abstract:
A microfluidic device for mitochondria analysis includes an inlet coupled to a first access channel, an outlet coupled to a second access channel, and a plurality of trapping channels fluidically coupled at one end to the first access channel and fluidically coupled at an opposing end to the second access channel, each trapping channel comprises a cross-sectional dimension about 2 μm in one direction and a cross-sectional dimension between about 0.45 and about 0.75 μm in a second direction.
Device And Method For Mitochondrial Membrane Potential Assessment
Peter Burke - Irvine CA, US Tae-Sun Lim - Hillsboro OR, US Antonio Davila - Philadelphia PA, US Douglas C. Wallace - Swarthmore PA, US Katayoun Zand - Irvine CA, US
International Classification:
G01N 27/26 B32B 37/02 G01N 27/333
US Classification:
205792, 204415, 204412, 156281
Abstract:
A microfluidic sensor device includes a substrate having patterned thereon at least one Ag/AgCl electrode (working electrode) and an inner chamber overlying the at least one Ag/AgCl electrode. The device includes an ion selective permeable membrane permeable to TPP disposed on one side of the first chamber and a sensing chamber overlying the ion selective permeable membrane. A separate reference electrode is inserted into the sensing chamber. The working electrode and reference electrode are coupled to a voltmeter to measure voltage. This voltage can then be translated into a TPP concentration which is used to determine the mitochondrial membrane potential (ΔΨ).