Arlene D Leon

US Patent:

20040180325, Sep 16, 2004

Filed:

Mar 26, 2004

Appl. No.:

10/810995

Inventors:

David Edwards - Addison TX, US
Arlene Leon - Garland TX, US
David Ranney - Dallas TX, US

International Classification:

C12Q001/00
C12N015/74

US Classification:

435/004000, 435/483000

Abstract:

A method for detecting interactions between first and second interacting molecules a variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.

US Patent:

6790607, Sep 14, 2004

Filed:

Oct 6, 2000

Appl. No.:

09/680738

Inventors:

David N. Edwards - Addison TX
Arlene Leon - Garland TX
David F. Ranney - Dallas TX

Assignee:

Hybrigen, Inc. - Dallas TX

International Classification:

C12Q 100

US Classification:

435 4, 435 691, 435 697, 435 711, 435 6

Abstract:

A method for detecting interactions between first and second interacting molecules at variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.