Arnon P Krongrad

US Patent:

52502848, Oct 5, 1993

Filed:

Oct 15, 1991

Appl. No.:

7/776569

Inventors:

Arnon Krongrad - Surfside FL

International Classification:

G01N 2408
A61K 3332

US Classification:

424 9

Abstract:

A method of enhancing a magnetic resonance signal comprising the steps of administering a quantity of a selected magnetic isotope to a living biological tissue at a concentration greater than the naturally occurring concentration of such isotope and detecting magnetic resonance signal from the administered magnetic isotope in the living biological tissue.

US Patent:

57863628, Jul 28, 1998

Filed:

Jun 16, 1994

Appl. No.:

8/260884

Inventors:

Arnon Krongrad - Surfside FL

Assignee:

University of Miami - Miami FL

International Classification:

A61K 3144

US Classification:

514280

Abstract:

Activation of the c-fos promoter and the serum response element (SRE) in androgen independent cancer cells has been demonstrated. In androgen dependent cells, direct activation of protein kinase C (PKC) or serum response factor (SRF) activated the c-fos promoter or SRE and caused androgen independent expression of an androgen target promoter. The highly specific PKC inhibitor chelerythrine selectively killed androgen independent cells. Expression of mutant SRF cDNA's inhibited activation of the SRE in prostate cancer cells. These reagents demonstrate the feasibility of both chemotherapy and gene therapy in interfering with intracellular pathways on which androgen independent cells are dependent; i. e. a strategy is outlined for treating hormone independent cancer in which the PKC-SRE pathway is disrupted by either chemotherapy or gene therapy.