Karl O. Voss - Foster City CA, US Arunashree Bhamidipati - Palo Alto CA, US
International Classification:
G01N 33/53
US Classification:
435 71
Abstract:
The present invention relates to methods and reagents for quantifying analytes. More specifically, the present invention related to methods and reagents for measuring one or more analytes present in a sample.
Tom Weisan Yang - Cupertino CA, US Arunashree Bhamidipati - Palo Alto CA, US Andrei V. Bordunov - Campbell CA, US James Eugene Knittle - San Jose CA, US Roger A. O'Neill - San Carlos CA, US Karl O. Voss - Foster City CA, US
International Classification:
G01N 33/53 G01N 27/26
US Classification:
436501, 204453, 422 681, 204601
Abstract:
Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
Tom Weisan Yang - Cupertino CA, US Arunashree Bhamidipati - Palo Alto CA, US Andrei V. Bordunov - Campbell CA, US James Eugene Knittle - San Jose CA, US Roger A. O'Neill - San Carlos CA, US Karl O. Voss - Foster City CA, US
Assignee:
Cell Biosciences, Inc. - Palo Alto CA
International Classification:
G01N 27/447
US Classification:
204451, 204450, 204600, 204601
Abstract:
Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
Thang Pham - Mountain View CA, US Arunashree Bhamidipati - Palo Alto CA, US Kevin Travers - Menlo Park CA, US Eric Olivares - Union City CA, US Tyson A. Clark - Menlo Park CA, US Jonas Korlach - Newark CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68 C40B 20/00 C40B 30/04 C12P 19/34
US Classification:
506 2, 435 915, 435 611, 435 9152, 506 9
Abstract:
Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released.
Thang Pham - Mountain View CA, US Jeremy Gray - San Francisco CA, US Adrian Fehr - San Francisco CA, US AJ Chmura - Oakland CA, US Yu-Chih Tsai - Mountain View CA, US Arunashree Bhamidipati - Palo Alto CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C40B 50/06 C40B 60/14
US Classification:
506 26, 506 40
Abstract:
Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.
- Menlo Park CA, US Arunashree Bhamidipati - Palo Alto CA, US Kevin Travers - Menlo Park CA, US Eric Olivares - Union City CA, US Tyson A. Clark - Menlo Park CA, US Jonas Korlach - Camas WA, US
International Classification:
C12Q 1/6874 C12Q 1/6869 G01N 33/543 B01L 9/00
Abstract:
Compositions, methods and systems are provided for isolating nucleic acids. A polymerase-nucleic acid complex can be formed by mixing a polymerase enzyme comprising strand displacement activity and a mixture of double stranded nucleic acids. Nucleic acid synthesis can then be initiated by the polymerase enzyme to produce a nascent strand complementary to the first strand, thereby displacing a portion of the second strand. After halting or reducing the rate of nucleic acid synthesis, a hybridizing a hook oligonucleotide can be used hybridize to the nucleic acid through a capture region on the hook oligonucleotide that is complementary to the displaced portion of the second strand. The nucleic acid can then be isolated from the mixture of nucleic acids using the hook oligonucleotide.
- Menlo Park CA, US Arunashree Bhamidipati - Palo Alto CA, US Kevin Travers - Menlo Park CA, US Eric Olivares - Union City CA, US Tyson A. Clark - Menlo Park CA, US Jonas Korlach - Camas WA, US
International Classification:
C12Q 1/68 G01N 33/543 B01L 9/00
Abstract:
Compositions, methods and systems are provided for isolating DNA having a modified or unnatural base. Circular DNA fragments, each comprising a double stranded DNA central region and single stranded regions on the ends of the double stranded regions, are obtained. Some of the fragments have one or more modified or unnatural base. The DNA fragments are treated with a primer and a polymerase such that the polymerase extends the primer to copy at least one of the strand of the double stranded region. This results in rendering the other strand single stranded. A binding protein or antibody that is specific to the modified or unnatural base is then used to isolate strands containing the modified or unnatural bases. Methods for loading such complexes onto substrates and for single molecule sequencing of such complexes are also provided.
- Menlo Park CA, US Jeremy Gray - Larkspur CA, US Adrian Fehr - San Francisco CA, US Aj Chmura - Oakland CA, US Yu-Chih Tsai - Fremont CA, US Arunashree Bhamidipati - Palo Alto CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
B01L 9/00
US Classification:
422561
Abstract:
Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.