Badri Prasad Maliwal

US Patent:

7842491, Nov 30, 2010

Filed:

Nov 8, 2004

Appl. No.:

10/982956

Inventors:

Richard B. Thompson - Baltimore MD, US
Daniel Elbaum - Newton MA, US
Vincent L. Feliccia - Arnold MD, US
David Christianson - Media PA, US
Marcia W. Patchan - Columbia MD, US
Zhengfang Ge - Rockville MD, US
Badri P. Maliwal - San Francisco CA, US

International Classification:

C12Q 1/34
C12Q 1/00
G01N 21/00
G01N 33/20

US Classification:

435232, 436 81, 435975, 435 18

Abstract:

The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.

US Patent:

20020055091, May 9, 2002

Filed:

Aug 31, 2001

Appl. No.:

09/942708

Inventors:

Richard Thompson - Baltimore MD, US
Daniel Elbaum - Newton MA, US
Vincent Feliccia - Arnold MD, US
David Christianson - Media PA, US
Marcia Patchan - Columbia MD, US
Zhengfang Ge - Burlington MA, US
Badri Maliwal - Baltimore MD, US

Assignee:

University of Pennsylvania

International Classification:

G01N033/20

US Classification:

435/004000, 436/074000, 436/079000, 436/080000, 436/081000, 436/083000, 436/084000, 436/166000, 436/172000

Abstract:

Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.

US Patent:

52468674, Sep 21, 1993

Filed:

Jan 17, 1992

Appl. No.:

7/822382

Inventors:

Joseph R. Lakowicz - Columbia MD
Badri P. Maliwal - Baltimore MD
Peter A. Koen - Hillsborough NJ

Assignee:

University of Maryland at Baltimore - MD
Becton Dickinson & Company - NJ

International Classification:

G01N 2164
G01N 3366

US Classification:

436 95

Abstract:

A method for measuring the concentration of a saccharide, conjugated saccharide or polysaccharide of interest using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is added to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent. The acceptor is bound to a carrier, while the donor and any saccharide, conjugated saccharide or polysaccharide of interest present in the sample compete for binding sites on the carrier. The sample is irradiated and the resultant emission detected. Energy transfer occurs between the donors and the acceptors, which produces a detectable lifetime change of the fIuorescence of the donor. The lifetime change is reduced or even eliminated by the competitive binding of a saccharide, conjugated saccharide or polysaccharide of interest to the donor. By measuring the apparent luminescent lifetime, the amount of a saccharide, conjugated saccharide or polysaccharide of interest in the sample can be determined.

US Patent:

62845446, Sep 4, 2001

Filed:

Apr 30, 1998

Appl. No.:

9/071351

Inventors:

Richard B. Thompson - Baltimore MD
Daniel Elbaum - Newton MA
Vincent L. Feliccia - Arnold MD
David Christianson - Media PA
Marcia W. Patchan - Columbia MD
Zhengfang Ge - Burlington MA
Badri P. Maliwal - Baltimore MD

Assignee:

University of Pennsylvania - Philadelphia PA
University of Maryland - Baltimore MD

International Classification:

G01N 3320

US Classification:

436 73

Abstract:

Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions.

US Patent:

61972584, Mar 6, 2001

Filed:

Mar 19, 1999

Appl. No.:

9/273303

Inventors:

Richard B. Thompson - Baltimore MD
Vincent L. Feliccia - Arnold MD
Badri P. Maliwal - Baltimore MD
Carol A. Fierke - Durham NC

Assignee:

University of Maryland, Baltimore - Baltimore MD

International Classification:

G01N 3320
C12Q 100

US Classification:

422 8207

Abstract:

The invention described in detail herein relates to the detection, determination, and quantitation of certain ions and small molecules in solution. The invention specifically relates to improvements in the area of photoluminescent sensors for use in a detection scheme involving the alteration of a photoluminescent label or moiety attached to or associated with an analyte binding macromolecule. One may use the changes in photoluminescence lifetime, changes in ratios of photoluminescence intensity or changes in photoluminescence polarization (anisotropy) to determine the analyte. The photoluminescence change measured correlates to the concentration of the ion or molecule in solution.

US Patent:

56311699, May 20, 1997

Filed:

Jan 19, 1994

Appl. No.:

8/183238

Inventors:

Joseph R. Lakowicz - Columbia MD
Badri P. Maliwal - Baltimore MD
Richard Thompson - Baltimore MD
Alvydas Ozinskas - Dayton MD

International Classification:

G01N 33533

US Classification:

436537

Abstract:

A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.

US Patent:

20140274797, Sep 18, 2014

Filed:

Mar 12, 2014

Appl. No.:

14/206738

Inventors:

- Fort Worth TX, US
Rafal Fudala - Fort Worth TX, US
Badri P. Maliwal - Fort Worth TX, US

International Classification:

C12Q 1/37

US Classification:

506 11, 530300

Abstract:

The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.