Tcu Nov 2013 - Dec 2014
Consultant
University of North Texas Apr 2010 - Aug 2014
Visiting Research Assistant Professor, on and Off
University of Maryland Medical School 1981 - 2002
Research Associate and Research Assistant Professor
University of Delaware Jun 1986 - May 1987
Visiting Research Scientist
North Carolina State University Oct 1978 - Aug 1981
Post-Doc
Skills:
Fluorescence Biochemistry Spectroscopy Protein Chemistry Microscopy Life Sciences Cell Biology Science Bioanalytical Chemistry Assay Development Biophysics Ligand Binding Cell Confocal Microscopy Biotechnology Research Fluorescence Microscopy
Interests:
Civil Rights and Social Action
Us Patents
Determination Of Metal Ions In Solution By Photoluminescence Anisotropy
Richard B. Thompson - Baltimore MD, US Daniel Elbaum - Newton MA, US Vincent L. Feliccia - Arnold MD, US David Christianson - Media PA, US Marcia W. Patchan - Columbia MD, US Zhengfang Ge - Rockville MD, US Badri P. Maliwal - San Francisco CA, US
International Classification:
C12Q 1/34 C12Q 1/00 G01N 21/00 G01N 33/20
US Classification:
435232, 436 81, 435975, 435 18
Abstract:
The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.
Determination Of Metal Ions In Solution By Photoluminescence Anisotropy
Richard Thompson - Baltimore MD, US Daniel Elbaum - Newton MA, US Vincent Feliccia - Arnold MD, US David Christianson - Media PA, US Marcia Patchan - Columbia MD, US Zhengfang Ge - Burlington MA, US Badri Maliwal - Baltimore MD, US
Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.
Determination And Quantification Of Saccharides By Luminescence Lifetimes And Energy Transfer
Joseph R. Lakowicz - Columbia MD Badri P. Maliwal - Baltimore MD Peter A. Koen - Hillsborough NJ
Assignee:
University of Maryland at Baltimore - MD Becton Dickinson & Company - NJ
International Classification:
G01N 2164 G01N 3366
US Classification:
436 95
Abstract:
A method for measuring the concentration of a saccharide, conjugated saccharide or polysaccharide of interest using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is added to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent. The acceptor is bound to a carrier, while the donor and any saccharide, conjugated saccharide or polysaccharide of interest present in the sample compete for binding sites on the carrier. The sample is irradiated and the resultant emission detected. Energy transfer occurs between the donors and the acceptors, which produces a detectable lifetime change of the fIuorescence of the donor. The lifetime change is reduced or even eliminated by the competitive binding of a saccharide, conjugated saccharide or polysaccharide of interest to the donor. By measuring the apparent luminescent lifetime, the amount of a saccharide, conjugated saccharide or polysaccharide of interest in the sample can be determined.
Determination Of Metal Ions In Solution By Photoluminescence Anisotropy
Richard B. Thompson - Baltimore MD Daniel Elbaum - Newton MA Vincent L. Feliccia - Arnold MD David Christianson - Media PA Marcia W. Patchan - Columbia MD Zhengfang Ge - Burlington MA Badri P. Maliwal - Baltimore MD
Assignee:
University of Pennsylvania - Philadelphia PA University of Maryland - Baltimore MD
International Classification:
G01N 3320
US Classification:
436 73
Abstract:
Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions.
Richard B. Thompson - Baltimore MD Vincent L. Feliccia - Arnold MD Badri P. Maliwal - Baltimore MD Carol A. Fierke - Durham NC
Assignee:
University of Maryland, Baltimore - Baltimore MD
International Classification:
G01N 3320 C12Q 100
US Classification:
422 8207
Abstract:
The invention described in detail herein relates to the detection, determination, and quantitation of certain ions and small molecules in solution. The invention specifically relates to improvements in the area of photoluminescent sensors for use in a detection scheme involving the alteration of a photoluminescent label or moiety attached to or associated with an analyte binding macromolecule. One may use the changes in photoluminescence lifetime, changes in ratios of photoluminescence intensity or changes in photoluminescence polarization (anisotropy) to determine the analyte. The photoluminescence change measured correlates to the concentration of the ion or molecule in solution.
Joseph R. Lakowicz - Columbia MD Badri P. Maliwal - Baltimore MD Richard Thompson - Baltimore MD Alvydas Ozinskas - Dayton MD
International Classification:
G01N 33533
US Classification:
436537
Abstract:
A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.
- Fort Worth TX, US Rafal Fudala - Fort Worth TX, US Badri P. Maliwal - Fort Worth TX, US
International Classification:
C12Q 1/37
US Classification:
506 11, 530300
Abstract:
The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.
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