Benjamin Franklin Cravatt

US Patent:

6699682, Mar 2, 2004

Filed:

Jun 28, 2001

Appl. No.:

09/894790

Inventors:

Norton B. Gilula - La Jolla CA
Benjamin F. Cravatt - San Diego CA
Richrd A. Lerner - La Jolla CA

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12Q 134

US Classification:

435 18, 435228, 435227, 435129, 436 34, 436 37

Abstract:

The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.

US Patent:

6872574, Mar 29, 2005

Filed:

Apr 16, 2001

Appl. No.:

09/836148

Inventors:

Benjamin F. Cravatt - La Jolla CA, US
Erik Sorensen - San Diego CA, US
Matthew P. Patricelli - San Diego CA, US
Martha Lovato - San Diego CA, US
Gregory Adam - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

G01N033/50
C11Q001/32
C12Q001/34

US Classification:

436119, 436 86, 436103, 436149, 436172, 435 71, 435 72, 435 18, 435 26

Abstract:

The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method is provided for generating and screening compound libraries that are used for the identification of lead molecules, and for the parallel identification of their biological targets. By appending specific functionalities and/or groups to one or more binding moieties, the reactive functionalities gain binding affinity and specificity for particular proteins and classes of proteins. Such libraries of candidate compounds, referred to herein as activity-based probes, or ABPs, are used to screen for one or more desired biological activities or target proteins.

US Patent:

7348173, Mar 25, 2008

Filed:

Feb 26, 2004

Appl. No.:

10/788992

Inventors:

Norton B. Gilula - La Jolla CA, US
Benjamin F. Cravatt - San Diego CA, US
Richrd A. Lerner - La Jolla CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12N 9/78
C12N 9/80
C12N 15/57

US Classification:

435228, 435 691, 435227, 4353201, 536 232, 514 44

Abstract:

The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.

US Patent:

7348437, Mar 25, 2008

Filed:

Jun 1, 2005

Appl. No.:

11/143009

Inventors:

Benjamin F. Cravatt - La Jolla CA, US
Alan Saghatelian - San Diego CA, US
Nadim Jessani - Los Altos CA, US
Arul Joseph - Cambridge MA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C07D 249/04
C07C 233/30
C07C 233/34
A61K 31/4192
A61K 31/191
A61K 31/192
A61K 31/194
A61K 31/195

US Classification:

548255, 514359, 514575, 564123

Abstract:

Activity-based compositions for analyzing metalloproteases are disclosed, where the compositions include a chemical compound including a hydroxamate moiety and a benzophenone moiety. Methods for synthesizing these compounds are also disclosed, as well as methods of using them for determining the bioactivity of a compositions comprising active compounds toward a metalloproteases and for determining the potency of an inhibitor against a metalloprotease.

US Patent:

7351874, Apr 1, 2008

Filed:

Jul 30, 2002

Appl. No.:

10/209002

Inventors:

Benjamin F. Cravatt - La Jolla CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

G01N 33/00
A01K 67/027

US Classification:

800 3, 800 18

Abstract:

The invention relates to an animal model for studying behavior related to fatty acid amide and hydrolysis of fatty acid amide. The invention provides transgenic animals in which the protein fatty acid amide hydrolase is not expressed, and methods of using such animals.

US Patent:

20020045194, Apr 18, 2002

Filed:

Dec 15, 2000

Appl. No.:

09/738954

Inventors:

Benjamin Cravatt - La Jolla CA, US
Erik Sorensen - San Diego CA, US
Matthew Patricelli - San Diego CA, US
Martha Lovato - San Diego CA, US
Gregory Adam - San Diego CA, US

International Classification:

G01N033/53

US Classification:

435/007900

Abstract:

The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method is provided for generating and screening compound libraries that are used for the identification of lead molecules, and for the parallel identification of their biological targets. By appending specific functionalities and/or groups to one or more binding moieties, the reactive functionalities gain binding affinity and specificity for particular proteins and classes of proteins. Such libraries of candidate compounds, referred to herein as activity-based probes, or ABPs, are used to screen for one or more desired biological activities or target proteins.

US Patent:

20020064799, May 30, 2002

Filed:

Apr 16, 2001

Appl. No.:

09/836145

Inventors:

Benjamin Cravatt - La Jolla CA, US
Erik Sorensen - San Diego CA, US
Matthew Patricelli - San Diego CA, US
Martha Lovato - San Diego CA, US
Gregory Adam - San Diego CA, US

Assignee:

The Scripps Research Institute of an Assignment

International Classification:

G01N033/53
C07C317/10
C07D213/44
C07D207/04

US Classification:

435/007100, 546/339000, 548/570000, 568/025000

Abstract:

The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method is provided for generating and screening compound libraries that are used for the identification of lead molecules, and for the parallel identification of their biological targets. By appending specific functionalities and/or groups to one or more binding moieties, the reactive functionalities gain binding affinity and specificity for particular proteins and classes of proteins. Such libraries of candidate compounds, referred to herein as activity-based probes, or ABPs, are used to screen for one or more desired biological activities or target proteins.

US Patent:

20020182652, Dec 5, 2002

Filed:

May 29, 2002

Appl. No.:

10/158498

Inventors:

Benjamin Cravatt - La Jolla CA, US
Erik Sorensen - San Diego CA, US
Matthew Patricelli - San Diego CA, US
Martha Lovato - San Diego CA, US
Gregory Adam - San Diego CA, US

International Classification:

G01N033/53
G01N033/542
G01N033/543

US Classification:

435/007900, 436/518000

Abstract:

The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method is provided for generating and screening compound libraries that are used for the identification of lead molecules, and for the parallel identification of their biological targets. By appending specific functionalities and/or groups to one or more binding moieties, the reactive functionalities gain binding affinity and specificity for particular proteins and classes of proteins. Such libraries of candidate compounds, referred to herein as activity-based probes, or ABPs, are used to screen for one or more desired biological activities or target proteins.