Benjamin J Hall

US Patent:

6475489, Nov 5, 2002

Filed:

Jun 7, 1995

Appl. No.:

08/482156

Inventors:

William J. Rutter - San Francisco CA
Pablo D. T. Valenzuela - San Francisco CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

The Regents of the University of California

International Classification:

A61K 3929

US Classification:

4241891, 530403

Abstract:

The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBSAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAq particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.

US Patent:

6544757, Apr 8, 2003

Filed:

Jun 21, 1988

Appl. No.:

07/209504

Inventors:

William J. Rutter - San Francisco CA
Pablo D. T. Valenzuela - San Francisco CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

The Regents of the University of California

International Classification:

C12P 2106

US Classification:

435 691, 4353201, 4352542, 4352541, 536 2372

Abstract:

The present invention relates to synthesis of HB Ag in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HB Ag by yeast has been achieved. The product is antigenic (reactive with anti-HB Ag), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HB Ag synthesized by yeast has identical sedimentation behavior to purified, naturally occurring HB Ag particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.

US Patent:

47692383, Sep 6, 1988

Filed:

Dec 12, 1985

Appl. No.:

6/821146

Inventors:

William J. Rutter - San Francisco CA
Pablo D. T. Valenzuela - San Francisco CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

The Regents of the University of California - CA

International Classification:

A61K 3929
C12P 2102
C12N 1500
C12N 118

US Classification:

424 89

Abstract:

The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADHl, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBsAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.

US Patent:

58561234, Jan 5, 1999

Filed:

Jun 6, 1995

Appl. No.:

8/465986

Inventors:

Ronald A. Hitzeman - Pacifica CA
Franklin E. Hagie IV - Foster City CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

Washington Research Foundation - Seattle WA
Genentech, Inc. - South San Francisco CA

International Classification:

C12P 2100

US Classification:

435 691

Abstract:

DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.

US Patent:

59196513, Jul 6, 1999

Filed:

Jun 6, 1995

Appl. No.:

8/465927

Inventors:

Ronald A. Hitzeman - Pacifica CA
Franklin E. Hagie - Foster City CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

Washington Research Foundation - Seattle WA
Genentech, Inc. - South San Francisco CA

International Classification:

C12P 2100

US Classification:

435 691

Abstract:

DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.

US Patent:

58540180, Dec 29, 1998

Filed:

Jun 6, 1995

Appl. No.:

8/465931

Inventors:

Ronald A. Hitzeman - Pacifica CA
Franklin E. Hagie IV - Foster City CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

Washington Research Foundation - Seattle WA
Genentech, Inc. - South San Francisco CA

International Classification:

C12P 2100

US Classification:

435 691

Abstract:

DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.

US Patent:

56186762, Apr 8, 1997

Filed:

Jun 7, 1995

Appl. No.:

8/474333

Inventors:

Ronald A. Hitzeman - Pacifica CA
Franklin E. Hagie - Foster City CA
Benjamin D. Hall - Bellevue WA
Gustav Ammerer - Seattle WA

Assignee:

Genentech, Inc. - So. San Francisco CA
Washington Research Foundation - Seattle WA

International Classification:

C12N 116
C12N 1500
C12N 118
C12N 1511
C12P 2100
C12P 2102
C12P 2104
C12P 1934

US Classification:

435 691

Abstract:

A DNA expression vector capable, in a transformant strain of yeast, of expressing a biologically competent polypeptide ordinarily exogenous to yeast under the control of a genetically distinct yeast promoter, the polypeptide not being required for the growth of the transformant; the process of forming the transformant strain of yeast; and the transformant strain of yeast.