Bethany A Sutton

US Patent:

20140234979, Aug 21, 2014

Filed:

Nov 13, 2013

Appl. No.:

14/079330

Inventors:

- Carlsbad CA, US
Bethany Sutton - Eugene OR, US
George Hanson - Eugene OR, US
Timothy Updyke - Temecula CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

G01N 33/58

US Classification:

436 86

Abstract:

The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.