Bhupender S Gupta

US Patent:

20080293133, Nov 27, 2008

Filed:

May 14, 2008

Appl. No.:

12/153170

Inventors:

Lola M. Reid - Chapel Hill NC, US
Bhupender S. Gupta - Cary NC, US
Jeffrey M. MacDonald - Chapel Hill NC, US

International Classification:

C12M 3/00
C12M 1/00

US Classification:

4352941, 4353071

Abstract:

A bioreactor for three-dimensional culture of liver cells is disclosed. The device is characterized by the use of textile vasculatures. A model and method for optimizing vasculature parameters is also disclosed. Liver acinar structure and physiological parameters are mimicked by sandwiching cells in the space between the two innermost woven textile hollow fibers, and creating radial flow of media from an outer compartment, through the cell mass compartment, and to an inner compartment. The theoretical optimum hydraulic permeability for the two innermost semi-permeable membranes is determined based on physiological hepatic sinusoidal blood flow and pressures. Experimental studies using a flow rate and pressure monitoring systems in conjunction with phase-contrast velocity-encoded MRI confirm theoretical results. Novel woven vascular tubes with optimum hydraulic permeability are disclosed for culturing hepatocytes in the multi-coaxial bioreactor.

US Patent:

20040152149, Aug 5, 2004

Filed:

Oct 31, 2003

Appl. No.:

10/697870

Inventors:

Lola Reid - Chapel Hill NC, US
Bhupender Gupta - Cary NC, US
Jeffrey MacDonald - Chapel Hill NC, US

International Classification:

C12N005/00
C12M001/12

US Classification:

435/029000, 435/399000, 435/297400

Abstract:

A bioreactor for three-dimensional culture of liver cells is disclosed. The device is characterized by the use of textile vasculatures. A model and method for optimizing vasculature parameters is also disclosed. Liver acinar structure and physiological parameters are mimicked by sandwiching cells in the space between the two innermost woven textile hollow fibers, and creating radial flow of media from an outer compartment, through the cell mass compartment, and to an inner compartment. The theoretical optimum hydraulic permeability for the two innermost semi-permeable membranes is determined based on physiological hepatic sinusoidal blood flow and pressures. Experimental studies using a flow rate and pressure monitoring systems in conjunction with phase-contrast velocity-encoded MRI confirm theoretical results. Novel woven vascular tubes with optimum hydraulic permeability are disclosed for culturing hepatocytes in the multi-coaxial bioreactor.