Bill C Lin

US Patent:

20220280933, Sep 8, 2022

Filed:

May 23, 2022

Appl. No.:

17/751267

Inventors:

- Pleasanton CA, US
Hanyoup KIM - Foster City CA, US
Bill Kengli LIN - Pleasanton CA, US
Marissa PENNELL - San Ramon CA, US
Alireza SALMANZADEH - San Francisco CA, US
Martin SAUZADE - Pleasanton CA, US
Tobias Daniel WHEELER - Pleasanton CA, US

International Classification:

B01L 3/02
B01L 3/00

Abstract:

Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

US Patent:

20220195420, Jun 23, 2022

Filed:

Nov 2, 2021

Appl. No.:

17/517282

Inventors:

- Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Pleasanton CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Pranav Patel - Fremont CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6869

Abstract:

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

US Patent:

20230017773, Jan 19, 2023

Filed:

Jul 26, 2022

Appl. No.:

17/873586

Inventors:

- Pleasanton CA, US
Augusto Manuel Tentori - Dublin CA, US
Siyuan Xing - Newark CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Bill Kengli Lin - Pleasanton CA, US
Felice Alessio Bava - Pleasanton CA, US
Pratomo Putra Alimsijah - Pleasanton CA, US
Nabil Mikhaiel - Dublin CA, US

International Classification:

B01L 3/00
C12Q 1/6837
G01N 1/31
C12Q 1/6841

Abstract:

Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

US Patent:

20210372998, Dec 2, 2021

Filed:

Mar 22, 2021

Appl. No.:

17/209045

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

US Patent:

20210222158, Jul 22, 2021

Filed:

Mar 22, 2021

Appl. No.:

17/208369

Inventors:

- Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Pleasanton CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Pranav Patel - Fremont CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6869

Abstract:

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

US Patent:

20210102936, Apr 8, 2021

Filed:

Jun 9, 2020

Appl. No.:

16/897126

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

US Patent:

20200391211, Dec 17, 2020

Filed:

Apr 27, 2020

Appl. No.:

16/859060

Inventors:

- Pleasanton CA, US
Bill Kengli LIN - Pleasanton CA, US
Anthony MAKAREWICZ - Livermore CA, US
Rajiv BHARADWAJ - Pleasanton CA, US

International Classification:

B01L 3/00
C12Q 1/6869

Abstract:

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

US Patent:

20200290048, Sep 17, 2020

Filed:

Feb 21, 2020

Appl. No.:

16/797727

Inventors:

- Pleasanton CA, US
Anthony MAKAREWICZ - Livermore CA, US
Bill Kengli LIN - Pleasanton CA, US

International Classification:

B01L 3/00
B01F 11/02
B01F 13/00
B01F 3/08
B01L 3/02

Abstract:

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.