The present invention is a cigarette packet with openings on the package to avoid the user from touching the filter end of the cigarette to increase hygiene. Also shown is a package with dual openings to allow a user to open the packet from either end to make it more convenient to open the packet without having to flip the packet to the opening end.
Epitope-Tagged Recombinant Growth Arrest Specific Gene 6 Protein
Jing Yang - Ambler PA, US Cailin Chen - New Hope PA, US Bing Hu - Media PA, US Patricia Andrade-Gordon - Doylestown PA, US Robert Jordan - Thornton PA, US
International Classification:
A61K038/17 C07K014/705
US Classification:
514/012000, 530/350000
Abstract:
The present invention provides epitope-tagged mammalian recombinant Growth Arrest Specific Gene 6 (Gas6) proteins or polypeptides that retain their biological activity of Gas6, including a novel method of generating and purifying the aforesaid proteins or polypeptides. Such biolgically active eptitope-tagged Gas6 proteins or polypeptides can be used when the presence of Gas6 is required and/or when Gas6 antagonists and receptors need to be identified. Prior to being expressed from a mammalian cell, the recombinant Gas6 protein or polypeptide is tagged with either Flag or polyhistidine to the C-terminus. After expression, the protein or polypeptide is purified in a single step affinity purification using either anti-Flag antibody resin or nickle resin. The purified epitope-tagged mammalian recombinant Gas6 protein or polypeptide maintains its biological activity and can be used thereafter.
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Bing Hu Information Technology Manager
China America Holding Company (Inc) Holding Company