Brent Howard Hilbert

US Patent:

7888081, Feb 15, 2011

Filed:

May 23, 2007

Appl. No.:

11/752492

Inventors:

Anil Bhagwan Khare - Crystal MN, US
Brent H. Hilbert - South Haven MN, US
Christopher Solheid - Minneapolis MN, US
Sara C. McFarlan - St. Paul MN, US
Fernando A. Sanchez-Riera - Eden Prairie MN, US
Kent Floy - Minnetonka MN, US
Paula M. Hicks - Bend OR, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

C12P 13/22
C12N 1/21

US Classification:

435121, 435108, 435193, 435232, 4352523

Abstract:

Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

US Patent:

20030139610, Jul 24, 2003

Filed:

Jul 23, 2002

Appl. No.:

10/201191

Inventors:

Anil Khare - Crystal MN, US
Brent Hilbert - South Haven MN, US

International Classification:

C07D321/00

US Classification:

549/200000

Abstract:

The invention provides compositions and methods for isolating phenolic compounds, particularly isoflavones, from aqueous extracts of plant materials that contain such compounds. The process comprising: (a) providing an aqueous plant extract at a first pH less than 10, the aqueous plant extract comprising a plurality of phenolic compounds; (b) extracting the aqueous plant extract with a first organic solvent to yield a first organic extract; (c) extracting the first organic extract with an aqueous phase of pH greater than 10 to yield a phenol rich aqueous phase; (d) adjusting the pH of the phenol rich aqueous phase to a pH of less than 9; and (e) isolating the phenolic compounds from the phenol rich aqueous phase.

US Patent:

20040258829, Dec 23, 2004

Filed:

Apr 2, 2004

Appl. No.:

10/817643

Inventors:

Guo-Hua Zheng - Centerville OH, US
Richard Hess - Chanhassen MN, US
Anil Khare - Crystal MN, US
Brent Hilbert - South Haven MN, US
Matt Deguise - Wayzata MN, US

International Classification:

A23L001/05

US Classification:

426/615000

Abstract:

A dietary fiber material obtained by using enzymes to digest cereal grains. The enzymes substantially hydrolyze any starch occurring in the cereal grain into small molecules but only partially hydrolyze -glucan molecules. The dietary fiber material can have excellent physicochemical, physiological, and sensory properties such ass low molecular weight, a particular molecular weight distribution, and a particular polydispersity, in addition to having excellent food ingredient properties. As a food ingredient, the material can have high -glucan content which provides nutrient to the body. Because the dietary fiber material can have neutral mouthfeel, it can be used to enhance the nutritional content of ice cream, yogurt, baked goods, bars, beverages, or certain other foods—without affecting the taste or other sensory attributes of the food. As a further benefit, the dietary fiber material can be used to provide certain therapeutic benefits, such as anticholesterol activity.

US Patent:

20090198072, Aug 6, 2009

Filed:

Dec 5, 2008

Appl. No.:

12/315685

Inventors:

Anil B. Khare - Crystal MN, US
Brent H. Hilbert - South Haven MN, US
Christopher Solheid - Minneapolis MN, US
Sara C. McFarlan - St. Paul MN, US
Trent H. Pemble - St. Bonifacius MN, US

Assignee:

CARGILL, INCORPORATED - WAYZATA MN

International Classification:

C07D 209/20

US Classification:

548495

Abstract:

Methods and systems for increasing the production of monatin in a multi-step equilibrium pathway are described. In some embodiments, a method includes adding alanine to a reactor along with one or more reactants and one or more enzymes, and producing a mixture comprising monatin and one or more intermediates. The addition of alanine results in a decreased concentration of less stable intermediates and an increased concentration of monatin.

US Patent:

20120264948, Oct 18, 2012

Filed:

Dec 30, 2010

Appl. No.:

13/518965

Inventors:

Brent Howard Hilbert - Annandale MN, US
Trent H. Pemble - St. Bonifacius MN, US
Michael A. Porter - Maple Gove MN, US
Christopher Solheid - Minneapolis MN, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

C07D 209/20

US Classification:

548495

Abstract:

Methods and systems for separating and purifying monatin are described. In the production of monatin, a mixture is formed which includes monatin, starting materials and intermediates. The methods and systems include a membrane having a zeta potential of from about −19 to −6 such that the membrane rejects greater than about 90% of the monatin. In some embodiments, the membrane is a nanofiltration membrane.

US Patent:

20160206648, Jul 21, 2016

Filed:

Mar 29, 2016

Appl. No.:

15/084263

Inventors:

- Wayzata MN, US
Richard K. HESS - Chanhassen MN, US
Anil Bhagwan KHARE - Crystal MN, US
Brent Howard HILBERT - Annandale MN, US
Matthew L. DEGUISE - Denver CO, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

A61K 31/716
C12P 19/04
C12P 19/14

Abstract:

A dietary fiber material obtained by using enzymes to digest cereal grains. The enzymes substantially hydrolyze any starch occurring in the cereal grain into small molecules but only partially hydrolyze β-glucan molecules. The dietary fiber material can have excellent physicochemical, physiological, and sensory properties such as low molecular weight, a particular molecular weight distribution, and a particular polydispersity, in addition to having excellent food ingredient properties. As a food ingredient, the material can have high β-glucan content which provides nutrient to the body. Because the dietary fiber material can have neutral mouthfeel, it can be used to enhance the nutritional content of ice cream, yogurt, baked goods, bars, beverages, or certain other foods—without affecting the taste or other sensory attributes of the food. As a further benefit, the dietary fiber material can be used to provide certain therapeutic benefits, such as anticholesterol activity.