Brett C Isenberg

US Patent:

20210340477, Nov 4, 2021

Filed:

Apr 30, 2021

Appl. No.:

17/246028

Inventors:

- Cambridge MA, US
Hesham Azizgolshani - Belmont MA, US
Brian P. Cain - Cambridge MA, US
Brett C. Isenberg - Newton MA, US
Joseph L. Charest - Jamaica Plain MA, US
Else M. Vedula - Stoneham MA, US
Ashley L. Gard - Dorchester MA, US
Ryan S. Maloney - Cambridge MA, US
Jeffrey T. Borenstein - West Roxbury MA, US
Rebeccah Lulu - Cambridge MA, US

Assignee:

The Charles Stark Draper Laboratory Inc. - Cambridge MA

International Classification:

C12M 3/06
C12M 1/32
C12M 1/00
C12M 1/12

Abstract:

The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.

US Patent:

20210238526, Aug 5, 2021

Filed:

Apr 26, 2021

Appl. No.:

17/240634

Inventors:

- Cambridge MA, US
Joseph L. Charest - Cambridge MA, US
Else M. Vedula - Brookline MA, US
Jeffrey T. Borenstein - Newton MA, US
Abigail June Spencer - Boston MA, US
Brett C. Isenberg - West Newton MA, US

Assignee:

The Charles Stark Draper Laboratory, Inc. - Cambridge MA

International Classification:

C12M 1/12
C12M 1/32
C12M 3/06
C12M 1/00
G01N 33/50
C12N 5/071
C12M 1/36
C12M 3/00
C12M 1/34
C12M 1/42

Abstract:

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

US Patent:

20210162416, Jun 3, 2021

Filed:

Dec 1, 2020

Appl. No.:

17/108475

Inventors:

- Cambridge MA, US
Nathan Moore - Canton MA, US
Daniel Doty - Arlington MA, US
Timothy Haggerty - Concord MA, US
Joseph Charest - Jamaica Plain MA, US
Alla Gimbel - Medford MA, US
Vienna Mott - Cambridge MA, US
Brett Isenberg - Newton MA, US
Hesham Azizgolshani - Belmont MA, US
Brian Cain - Cambridge MA, US
Mark Mescher - West Newton MA, US

International Classification:

B01L 3/00
G01N 33/50

Abstract:

A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.

US Patent:

20210071125, Mar 11, 2021

Filed:

Sep 4, 2020

Appl. No.:

17/013454

Inventors:

- Cambridge MA, US
Brian Cain - Cambridge MA, US
Joseph Charest - Cambridge MA, US
Jonathan Robert Coppeta - Windham NH, US
Brett Isenberg - Newton MA, US
Timothy Petrie - Braintree MA, US

International Classification:

C12M 3/06
C12M 1/26
C12M 1/12

Abstract:

This disclosure provides systems and methods for seeding cell cultures in a microfluidic device. The systems and methods of this disclosure can enable flow of a cell solution from one side of a scaffold, such as a porous substrate or membrane, to the other side of the scaffold. Flow of the liquid can pass through the scaffold while the cells themselves do not, resulting in the cells driven to the surface of the scaffold for consequent attachment. A microfluidic device can include a microfluidic feature structured to create a seal between a cell seeding tool and an inlet to a microchannel of the microfluidic device. This can enable a pressure-driven flow to push fluid down the channel and through pores of the membrane. In contrast, traditional gravity fed seeding of cells may not create enough pressure to drive fluid through the pores of the scaffold.

US Patent:

20180142196, May 24, 2018

Filed:

Nov 21, 2017

Appl. No.:

15/819986

Inventors:

- Cambridge MA, US
Joseph L. Charest - Cambridge MA, US
Else M. Vedula - Brookline MA, US
Jeffrey T. Borenstein - Newton MA, US
Abigail June Spencer - Boston MA, US
Brett C. Isenberg - West Newton MA, US

International Classification:

C12M 1/12
C12M 1/32
C12M 3/06
C12M 1/00
C12M 1/36
G01N 33/50
C12N 5/071

Abstract:

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

US Patent:

20170117235, Apr 27, 2017

Filed:

Oct 21, 2016

Appl. No.:

15/331501

Inventors:

- Cambridge MA, US
Gregory M. Fritz - Wakefield MA, US
Jonathan R. Coppeta - Windham NH, US
Brett C. Isenberg - Newton MA, US

Assignee:

The Charles Stark Draper Laboratory Inc. - Cambridge MA

International Classification:

H01L 23/00

Abstract:

A destroy on-demand electrical device includes a substrate layer formed using a soluble material (e.g., a Germanium oxide), a semi-conductor layer formed from a material that can become soluble upon further processing (e.g., Germanium) and conductive elements, formed from a metallic material such as Copper. The device is coupled with one or more disintegration sources that contain disintegration agents (e.g., Hydrogen Peroxide) that can promote disintegration of the device. The device can be destroyed in response to actuation of the disintegration sources, for example by actuation of a source that produces Hydrogen Peroxide for use in oxidizing the semi-conductor layer. Water can be used to dissolve dissolvable substrate layers. The semi-conductor layer can be destroyed by first processing this layer to form a dissolvable material and dissolving the processed layer with water. The remaining Copper components disintegrate once their underlying layer have been dissolved and/or by use of a salt.

US Patent:

20160220997, Aug 4, 2016

Filed:

Feb 4, 2016

Appl. No.:

15/016227

Inventors:

- Cambridge MA, US
Jonathan Robert Coppeta - Windham NH, US
Abigail June Spencer - Boston MA, US
Brett Isenberg - Newton MA, US

International Classification:

B01L 3/00
C12M 3/06
C12M 3/00
F16K 7/12

Abstract:

Systems and methods disclosed herein related to an apparatus including a fluid flow plate and a microfluidic valve assembly. The fluid flow plate includes a plurality of polymer layers that define a fluid flow passage through the microfluidic valve assembly. The microfluidic valve assembly includes a valve seat, a flexible membrane, a valve cavity, a valve head, and an actuator. The actuator is configured to selectively control pressure applied by the valve head to the flexible membrane, such that in a first actuator state the valve head depresses the flexible membrane into the valve cavity and into contact with the valve seat, thereby preventing fluid flow through the valve assembly, and in a second state, the valve head and the flexible membrane are retracted substantially out of the valve cavity allowing fluid to flow through the valve assembly. In various implementations, the valve seat and/or the flexible membrane include an elastomer layer.

US Patent:

20160040112, Feb 11, 2016

Filed:

Aug 5, 2015

Appl. No.:

14/819358

Inventors:

- Cambridge MA, US
Brett Isenberg - Newton MA, US
Mark Mescher - West Newton MA, US

Assignee:

The Charles Stark Draper Laboratory, Inc. - Cambridge MA

International Classification:

C12M 1/02
C12M 1/36
C12M 1/00

Abstract:

Fluid circulation and leveling systems and methods of using the same are described. A fluid circulation system includes a fluid mixing chamber and open fluid chambers in fluid communication with the fluid mixing chamber. Each open fluid chamber includes a microfluidic fluid leveling conduit with an orifice disposed in the open fluid chamber at a minimum fluid level associated with a corresponding minimum fluid volume. A controller causes a first pump to generate a first direction of fluid flow during a first time period between the open fluid chambers, and causes the first pump to generate a second direction of fluid flow during a second time period between the first and second open fluid chambers. The controller also causes a second pump to generate a flow of fluid during a third time period from one of the first and second open fluid chambers into the fluid mixing chamber.