Brian Albarran

US Patent:

20060281677, Dec 14, 2006

Filed:

Jun 10, 2005

Appl. No.:

11/150588

Inventors:

Brian Albarran - Seattle WA, US
Patrick Stayton - Seattle WA, US
Richard To - Bellevue WA, US

Assignee:

University of Washington - Seattle WA

International Classification:

A61K 38/16
A61K 38/17
A61K 31/4188

US Classification:

514012000, 514393000

Abstract:

The invention provides compositions for intracellular delivery of biotinylated cargo. The compositions comprise a complex formed between (a) a fusion protein comprising a protein transduction domain linked to streptavidin and (b) a biotinylated cargo for intracellular delivery. In some embodiments, the protein transduction domain comprises the protein transduction domain of the Human Immunodeficiency Virus type 1 (HIV-1) TAT protein. The complex may further comprise a biotinylated endosomal releasing polymer, such as a poly(propylacrylic acid) polymer. The invention also provides methods for obtaining intracellular delivery of biotinylated cargo and methods for obtaining cytoplasmic delivery of biotinylated cargo.