Brian T Collins

US Patent:

8338088, Dec 25, 2012

Filed:

Apr 15, 2008

Appl. No.:

12/595902

Inventors:

Brian Edward Collins - Arlington MA, US
Carlos J. Bosques - Arlington MA, US
Xiangping Zhu - North Grafton MA, US
Dorota A. Bulik - Malden MA, US
Ian Christopher Parsons - Belmont MA, US
Zachary Shriver - Cambridge MA, US
Rajeev Chillakuru - Cambridge MA, US
Ganesh Venkataraman - Bedford MA, US

Assignee:

Momenta Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 1/00

US Classification:

435 4

Abstract:

The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins.

US Patent:

8361705, Jan 29, 2013

Filed:

Apr 15, 2008

Appl. No.:

12/595925

Inventors:

Ian Christopher Parsons - Belmont MA, US
Dorota A. Bulik - Malden MA, US
Carlos J. Bosques - Arlington MA, US
Brian Edward Collins - Arlington MA, US

Assignee:

Momenta Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 1/00

US Classification:

435 4, 435 14, 435183, 435195

Abstract:

The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.

US Patent:

20080270398, Oct 30, 2008

Filed:

Apr 30, 2007

Appl. No.:

11/796886

Inventors:

Matthew J. Landau - Arlington MA, US
Joseph Vigneau - Newton MA, US
Brian Collins - Allston MA, US

International Classification:

G06Q 30/00
G06F 17/18

US Classification:

707 6, 705 27

Abstract:

The present invention relates to a computerized engine and method for using the same to determine and effect product sales to customers based on customer-driven affinities relating to the products. In some embodiments, the computerized engine includes hardware and software and uses customer or transaction data or product characteristics to calculate an output relating to the affinity. In some embodiments, the computerized engine provides an output indicative of the affinity. In some embodiments, the computerized engine is coupled to a database containing the customer or transaction data or product characteristics. The system and method can also determine a reduced affinity depending on a period of time elapsed between a pair of events being related, or as a function of the age of collected affinity data.

US Patent:

20100113294, May 6, 2010

Filed:

Apr 15, 2008

Appl. No.:

12/596171

Inventors:

Ganesh Venkataraman - Bedford MA, US
Brian Edward Collins - Somerville MA, US
Carlos J. Bosques - Cambridge MA, US
Dorota A. Bulik - Malden MA, US
Ian Christopher Parsons - Belmont MA, US
Zachary Shriver - Cambridge MA, US
Rajeev Chillakuru - Cambridge MA, US

Assignee:

MOMENTA PHARMACEUTICALS, INC. - Cambridge MA

International Classification:

C40B 30/02
C40B 40/10

US Classification:

506 8, 506 18

Abstract:

The invention provides methods, databases and systems for making glycoprotein products having defined properties.

US Patent:

20110213137, Sep 1, 2011

Filed:

Apr 15, 2008

Appl. No.:

12/595888

Inventors:

Carlos J. Bosques - Arlington MA, US
Ian Christopher Parsons - Belmont MA, US
Brian Edward Collins - Arlington MA, US
Hetal Sarvaiya - Quincy MA, US

Assignee:

MOMENTA PHARMACEUTICALS, INC. - CAMBRIDGE MA

International Classification:

C07B 59/00
G01N 30/72
C12Q 1/02

US Classification:

536 553, 436 94, 435 29

Abstract:

The present disclosure provides methods of making isotopically-labeled glycans or a mixture of glycans (e.g., a glycan preparation) via reductive amination. Such methods are useful, for example, in the determination of the relative amounts of a glycan species present in two or more glycoprotein preparations using mass spectroscopy. In one aspect, the present disclosure provides a method of making an isotopically-labeled glycan by a glycan with an amine and an isotopically-labeled reducing agent to provide an isotopically-labeled aminated glycan. In certain embodiments, the amine is an isotopically-labeled amine.

US Patent:

20120264927, Oct 18, 2012

Filed:

Apr 15, 2008

Appl. No.:

12/595934

Inventors:

Ian Christopher Parsons - Belmont MA, US
Carlos J. Bosques - Arlington MA, US
Brian Edward Collins - Arlington MA, US

International Classification:

C07H 1/00

US Classification:

536 53, 536 55, 536 553

Abstract:

Methods for labeling glycans that include a step of freeze-drying a labeled glycan preparation. The labeled glycan preparation is maintained in a substantially frozen state for the duration of the freeze-drying process.

US Patent:

20120277165, Nov 1, 2012

Filed:

Jun 4, 2010

Appl. No.:

13/375577

Inventors:

Brian E. Collins - Arlington MA, US
Dorota A. Bulik - Malden MA, US
Kevin Millea - Saugus MA, US

International Classification:

C12P 21/00
C12N 5/071
C12M 3/00
A61K 38/14
G01N 27/62
G01N 30/02
G01N 24/08
C12Q 3/00
C12P 21/08
C12Q 1/02

US Classification:

514 209, 435 703, 435325, 4352891, 435 29, 435 3

Abstract:

The present invention provides methods and materials useful for monitoring and regulating the glycosylation of glycoproteins that are recombinantly produced from cells. In particular, methods are provided for monitoring and regulating levels of cellular indicators which affect the level of fucosylation produced by cells.

US Patent:

20120329709, Dec 27, 2012

Filed:

May 25, 2010

Appl. No.:

13/322049

Inventors:

Brian Edward Collins - Arlington MA, US
Tiffany Guo - Lagrange OH, US
Kevin Millea - Saugus MA, US
Dorota A. Bulik - Malden MA, US

International Classification:

C12P 21/00
A61K 38/38
A61K 38/28
A61K 38/27
A61K 38/26
A61K 38/37
C07K 14/57
C07K 14/76
C07K 14/755
C07K 16/40
C07K 14/61
C07K 16/24
C07K 14/62
G01N 27/62
C12Q 1/68
C12N 1/21
C12N 1/19
C12N 5/10
C12N 1/15
C12N 9/50
C12N 9/24
C12N 9/02
C12N 9/40
C12N 9/82
C12N 9/22
A61K 38/02

US Classification:

514 64, 514 209, 514 152, 514 114, 514 113, 514 117, 514 11, 514 141, 530351, 530362, 530383, 5303873, 530397, 53038815, 530304, 435 691, 435 18, 435325, 435 61, 43525233, 43525423, 4352542, 43525421, 435348, 435419, 435412, 435414, 43525411, 435364, 435369, 435358, 435354, 435367, 435353, 435366, 435370, 435350, 435372, 4353723, 435219, 435200, 435189, 435208, 435229, 435199

Abstract:

The present invention provides methods and materials by which glycosylation of glycoproteins can be regulated. Methods include the monitoring and regulation of parameters such that a glycoprotein having a desired product quality is obtained.