Brian D Mariani

US Patent:

20050239055, Oct 27, 2005

Filed:

Apr 22, 2004

Appl. No.:

10/829474

Inventors:

Brian Mariani - Herndon VA, US

International Classification:

C12Q001/70
C12Q001/68
C07H021/04

US Classification:

435005000, 435006000, 536023720

Abstract:

The present invention provides methods, primers and probes for the detection of enteroviral nucleic acids in biological fluids and tissue. In the methods of the invention, at least a portion of enteroviral nucleic acid present in a biological sample suspected of containing an enterovirus is amplified and the amplified enteroviral nucleic acid is then detected. Detection may be accomplished by conventional separation techniques such as gel electrophoresis or by hybridization of at least a portion of a nucleotide probe comprising a nucleotide sequence complementary to the amplified enteroviral nucleic acid. Preferably, enteroviral RNA is detected in a biological sample using real-time PCR techniques that can detect the increasing presence of an amplification product while amplification occurs.

US Patent:

20060141481, Jun 29, 2006

Filed:

Dec 27, 2004

Appl. No.:

11/020676

Inventors:

Brian Mariani - Herndon VA, US

International Classification:

C12Q 1/70
C12Q 1/68

US Classification:

435006000, 435005000

Abstract:

The present invention provides methods, primers and probes for the detection of HSV nucleic acids in biological fluids and tissue. In the methods of the invention, at least a portion of HSV nucleic acid present in a biological sample suspected of containing an HSV-1 and/or HSV-2 is amplified and the amplified HSV nucleic acid is then detected. Detection may be accomplished by conventional separation techniques such as gel electrophoresis or by hybridization of at least a portion of a nucleotide probe comprising a nucleotide sequence complementary to the amplified HSV nucleic acid. Preferably, HSV DNA is detected in a biological sample using real-time PCR techniques that can detect the increasing presence of an amplification product while amplification occurs.

US Patent:

56541414, Aug 5, 1997

Filed:

Nov 18, 1994

Appl. No.:

8/342091

Inventors:

Brian D. Mariani - Philadelphia PA
Rocky S. Tuan - Chester Springs PA

Assignee:

Thomas Jefferson University - Philadelphia PA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A method of detecting a bacterial infection in a patient comprising obtaining a patient sample; obtaining a sample of nucleic acids from the patient sample; separating charged cellular components from the nucleic acids using a mixed bed ion exchange resin; amplifying bacterial nucleic acids in the sample; and detecting the presence or absence of amplified nucleic acids wherein the presence of amplified nucleic acids indicates a bacterial infection.

Address:

3179 Kinross Cir, Herndon, VA 20171

Phone:

7034817989

VIN:

1FMHK7D98CGA51199

Make:

FORD

Model:

EXPLORER

Year:

2012