Brian Stanely Seed

US Patent:

42869641, Sep 1, 1981

Filed:

Oct 12, 1979

Appl. No.:

6/084509

Inventors:

Brian S. Seed - Scarsdale NY

International Classification:

C08B 1120
C08B 1506
G01N 3122
G01N 3316

US Classification:

23230B

Abstract:

A process for preparing a derivative of an hydroxyl bearing substrate by reacting an hydroxyl bearing substrate with oxirane bearing molecules to produce an oxirane ring bearing substrate; and reacting the oxirane ring with a substituted primary aromatic amine selected from primary aromatic amine alcohols and primary aromatic amine thiols. Among the compositions so produced is a stable modified hydroxyl bearing substrate or hydroxyl bearing sheet having covalently bonded thereto primary aromatic amines which can be diazotized. The diazotized substrate selectively, covalently binds proteins, polypeptides, peptides, nucleic acids, RNA, single stranded DNA, and nucleic acid hybrids.