Brian J Smart

US Patent:

20200190129, Jun 18, 2020

Filed:

Aug 17, 2018

Appl. No.:

16/640005

Inventors:

- Santa Clara CA, US
Joel MYERSON - Berkeley CA, US
Brian SMART - Santa Clara CA, US

International Classification:

C07H 1/06
C07H 21/02

Abstract:

Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.

US Patent:

20200181124, Jun 11, 2020

Filed:

Feb 18, 2020

Appl. No.:

16/793808

Inventors:

- Santa Clara CA, US
Joel MYERSON - Berkeley CA, US
Brian SMART - Santa Clara CA, US

International Classification:

C07D 405/12
C07D 403/06
C07D 317/34
C07H 21/02

Abstract:

Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.

US Patent:

20160122800, May 5, 2016

Filed:

Nov 3, 2014

Appl. No.:

14/531563

Inventors:

- Loveland CO, US
Robert Ach - San Francisco CA, US
Mistuni Ghosh - Santa Clara CA, US
Brian Smart - San Jose CA, US

International Classification:

C12Q 1/68

Abstract:

Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.