Brian W Snyder

US Patent:

53994841, Mar 21, 1995

Filed:

Oct 8, 1991

Appl. No.:

7/773064

Inventors:

Bradley P. Boyer - Rochester NY
Paul B. Contestable - Rochester NY
Brian A. Snyder - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33569

US Classification:

435 732

Abstract:

A method has been developed for determining microorganisms associated with periodontal diseases which is highly sensitive and shows very low background and cross-reactivity among various closely related antigens. Antigen is extracted at relatively high pH, and either before or immediately after extraction, the antigen-containing specimen is mixed with a blocking composition having at least about 0. 2 weight percent of a non-immunoreactive blocking protein. The pH of the resulting mixture is kept high when contacted with the antibodies specific to the antigen of interest. The compositions and components needed for the assay can be supplied in a diagnostic test kit.

US Patent:

59167572, Jun 29, 1999

Filed:

Jul 17, 1996

Appl. No.:

8/683247

Inventors:

Paul B. Contestable - Rochester NY
John L. Daiss - Rochester NY
Holly L. Groth - Hamlin NY
Elizabeth A. Grogan - Rochester NY
Brian A. Snyder - Rochester NY

Assignee:

Johnson & Johnson Clinical Diagnostics, Inc. - Rochester NY

International Classification:

G01N 3353

US Classification:

435 771

Abstract:

Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, an immobilized reporter enzyme, an inhibitor antibody and a a water-soluble conjugate of the ligand and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme, but the antibodies affect enzymatic activity differently. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the generated signal to the target specific binding ligand.

US Patent:

50949624, Mar 10, 1992

Filed:

Jun 13, 1988

Appl. No.:

7/206236

Inventors:

Brian A. Snyder - Rochester NY
Elizabeth A. Grogan - Rochester NY
Richard C. Sutton - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33543

US Classification:

436518

Abstract:

A water-insoluble microporous article comprises a microporous substrate having first and second outer surfaces. Affixed to at least one of those surfaces is a stabilized specific binding reagent admixed with certain hydrophilic, neutral or positively-charged binder materials. Particularly useful binder materials include certain quaternary polymers, vinylpyrrolidone polymers and acrylamide polymers. In this mixture, the reagent exhibits improved keeping stability compared to similar reagents used without binder materials. The reagent comprises water-insoluble particles to which are attached receptor molecules to a target ligand. Substantially none of the reagent is entrapped within the microporous substrate. This article is useful for the detection of a target ligand in an assay involving the specific binding reaction of the ligand with corresponding receptor molecules, and can be included in a diagnostic test kit. It is particularly useful for the detection of Streptococcal antigen in a biological specimen when the receptor molecules are antibodies to that antigen.

US Patent:

53786297, Jan 3, 1995

Filed:

Jun 7, 1993

Appl. No.:

8/072029

Inventors:

Bradley P. Boyer - Rochester NY
Paul B. Contestable - Rochester NY
Brian A. Snyder - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33569

US Classification:

436 17

Abstract:

An aqueous wash composition has been found useful in methods for determination of specific binding ligands. The composition is buffered to a pH of less than or equal to 6 or greater than or equal to 9. It also includes as its essential component at least about 0. 1 weight percent of an anionic surfactant which is represented by the formula: [A-SO. sub. 3+y ]. sup. -(1+y) [X. sup. +m ]. sub. n wherein A is a hydrocarbon having a molecular weight of at least about 180, X. sup. +m is hydrogen or a monovalent or divalent cation, m is 1 or 2, is 0 or 1, and n is 1 or 2 provided that m and n are not both 2. Optionally and preferably, the wash composition also includes a nonimmunoreactive protein. This wash composition is particularly useful in methods for determination of microorganisms associated with periodontal diseases. Such methods can be of a variety of formats, but immunometric assays are particularly useful.

US Patent:

53745167, Dec 20, 1994

Filed:

Feb 24, 1989

Appl. No.:

7/315086

Inventors:

Richard C. Sutton - Rochester NY
Brent A. Burdick - Rochester NY
Susan J. Danielson - Rochester NY
Harold C. Warren - Rush NY
Brian A. Snyder - Rochester NY
Gregory J. McClune - Rochester NY
Annie L. Wu - Penfield NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33545
G01N 33546

US Classification:

435 5

Abstract:

Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

US Patent:

52485951, Sep 28, 1993

Filed:

Oct 8, 1991

Appl. No.:

7/774019

Inventors:

Bradley P. Boyer - Rochester NY
Paul B. Contestable - Rochester NY
Brian A. Snyder - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33569

US Classification:

435 732

Abstract:

An aqueous wash composition has been found useful in methods for determination of specific binding ligands. The composition is buffered to a pH of less than or equal to 6 or greater than or equal to 9. It also includes as its essential component at least about 0. 1 weight percent of an anionic surfactant which is represented by the formula: [A-SO. sub. 3+y ]. sup. -(1+y) [X. sup. +m ]. sub. n wherein A is a hydrocarbon having a molecular weight of at least about 180, X. sup. +m is hydrogen or a monovalent or divalent cation, m is 1 or 2, y is 0 or 1, and n is 1 or 2 provided that m and n are not both 2. Optionally and preferably, the wash composition also includes a nonimmunoreactive protein. This wash composition is particularly useful in methods for determination of microorganisms associated with periodontal diseases. Such methods can be of a variety of formats, but immunometric assays are particularly useful.

US Patent:

48471992, Jul 11, 1989

Filed:

Feb 27, 1987

Appl. No.:

7/019850

Inventors:

Brian A. Snyder - Rochester NY
Robert T. Belly - Ithaca NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

C12M 112
C12Q 104
C12Q 114

US Classification:

435 36

Abstract:

A test kit is used in an agglutination immunoassay to determine a multivalent immune species, such as Streptococcus A antigen, in a biological sample. The method includes contacting an aqueous solution of the species with an agglutination indicator reagent having receptor molecules reactive with the species to form an agglutinate of the reaction product of species and receptor. These receptor molecules are bound to polymeric particles which contain tracer molecules. The resulting agglutinate is captured on a microporous membrane which has an average pore size which is at least five times greater than the average diameter of the polymeric particles. Unagglutinated residual materials are washed through the membrane using a wash solution. This solution has a pH of from about 5 to about 10 and an ionic strength of at least about 0. 25. Tracer is then determined either in the agglutinate or in the residual materials.

US Patent:

54609461, Oct 24, 1995

Filed:

Apr 6, 1993

Appl. No.:

8/043246

Inventors:

Paul B. Contestable - Rochester NY
Bradley P. Boyer - Rochester NY
Brian A. Snyder - Rochester NY
Thomas R. Kissel - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33569

US Classification:

435 732

Abstract:

The signal generated in a specific binding assay wherein a peroxidase label is used to detect the resulting specific binding complex on a microporous filtration membrane can be modulated by contacting the signal forming reagents with a buffered solution of a hydroxamic acid or acyl hydrazine having the structure R--CO--NH--R' or an equivalent salt thereof, wherein R is aryl of 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms or cycloalkyl of 5 to 10 carbon atoms in the ring, and R' is hydroxy or amino. This solution can be provided in a diagnostic test kit for use in various methods to detect a specific binding ligand. The result is improved signal stability and lowered background after the use of a high pH wash solution in the assay.