Brian D Wolf

US Patent:

7553869, Jun 30, 2009

Filed:

Jul 28, 2006

Appl. No.:

11/460700

Inventors:

Peter T. Czerney - Weimar, DE
Surbhi Desai - Rockford IL, US
Frank G. Lehmann - Jena, DE
Zakir S. Murtaza - Vernon Hills IL, US
Bernd G. Schweder - Jena, DE
Matthias S. Wenzel - Jena, DE
Brian David Wolf - Machesney Park IL, US

Assignee:

Pierce Biotechnology, Inc. - Rockford IL

International Classification:

A01N 43/16
A61K 31/35
C07D 311/02

US Classification:

514456, 549285

Abstract:

Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.

US Patent:

7947506, May 24, 2011

Filed:

Jun 2, 2006

Appl. No.:

11/421883

Inventors:

Brian David Wolf - Machesney Park IL, US
Surbhi Desai - Rockford IL, US
Peter T. Czerney - Weimar, DE
Frank G. Lehmann - Jena, DE
Bernd G. Schweder - Jena, DE
Matthias S. Wenzel - Jena, DE

Assignee:

Pierce Biotechnology, Inc. - Rockford IL

International Classification:

G01N 33/00
G01N 33/58
C12Q 1/68
C09B 23/00
C09B 57/02

US Classification:

436 86, 546 66, 546 89, 546 99, 546148, 546176, 435 6, 435 71, 548121, 548148, 548159, 548217

Abstract:

A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

US Patent:

20160266065, Sep 15, 2016

Filed:

Feb 15, 2016

Appl. No.:

15/043816

Inventors:

Gregory John Kilmer - Winnebago IL, US
Brian David Wolf - Machesney Park IL, US
Scott Eugene Starwalt - Byron IL, US
Gary G. Judd - DeKalb IL, US
Brian Lynn Webb - Roscoe IL, US

Assignee:

PIERCE BIOTECHNOLOGY, INC. - ROCKFORD IL

International Classification:

G01N 27/447

Abstract:

A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

US Patent:

20140224655, Aug 14, 2014

Filed:

Feb 12, 2013

Appl. No.:

13/764934

Inventors:

Gregory John Kilmer - Winnebago IL, US
Brian David Wolf - Machesney Park IL, US
Scott Eugene Starwalt - Byron IL, US
Gary G. Judd - DeKalb IL, US
Brian Lynn Webb - Roscoe IL, US

International Classification:

G01N 27/447

US Classification:

204464, 204468

Abstract:

A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.