Bridgett L Steele

Metabolism Technical Expert

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Location:

265 Celo Knob Trl, Kernersville, NC

Industry:

Research

Work:

Syngenta
Metabolism Technical Expert
Knoell Usa
Team Leader
Jrf America Dec 2012 - Aug 2014
Scientist Ii
University of Calgary Jun 2010 - Nov 2012
Postdoctoral Fellow
University of North Carolina at Chapel Hill Oct 2003 - Dec 2009
Research Assistant

Education:

University of North Carolina at Chapel Hill 2003 - 2009
Doctorates, Doctor of Philosophy, Chemistry Northern Kentucky University 1999 - 2003
Bachelors, Bachelor of Science, Chemistry Bishop Brossart High School

Skills:

Tirf
Fluorescence Microscopy
Hplc
Gc
Sds Page
Western Blotting
Elisa
Sec Malls
Tissue Culture
Protein Purification
Confocal Microscopy
Gas Chromatography
Protein Chemistry
Enzyme Assays
Uv/Vis
Glp
Lc Ms
Analytical Chemistry
Mass Spectrometry
Fluorescence Spectroscopy
Chromatography
Gc Ms
Biochemistry

Interests:

Science and Technology

Bridgett Steele

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Bridgett Steele Audubon, PA

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Work:

JRF America

Dec 2012 to 2000
Scientist II, E-Fate and Metabolism Dr. Tannin Schmidt, University of Calgary

Jun 2010 to Nov 2012
Postdoctoral Fellow Dr. Nancy Thompson, University of North Carolina

Oct 2003 to Dec 2009
Ph.D. Candidate

Education:

University of North Carolina
Chapel Hill, NC
Dec 2009
Ph.D. in Analytical Chemistry Northern Kentucky University
Highland Heights, KY
May 2003
B.S. in Chemistry

Skills:

Analytical chemist with experience in separation of small molecules and large molecules, including proteins and peptides, using HPLC-UV and HPLC-Rad Radiation Safety training; Experience working with 14C labeled compounds Familiar with EPA and OECD guidelines for environmental fate studies Multi-angle laser light scattering (MALLS) for determination of molecular weight Protein purification using affinity column chromatography, ion exchange chromatography, size exclusion chromatography (SEC), and FPLC Knowledgeable in recombinant protein expression in bacterial (E. coli) cells including transformation, DNA amplification and quantification, protein production, and purification of tagged proteins Proficiency in total internal reflection fluorescence (TIRF) microscopy and confocal fluorescence recovery after photobleaching (FRAP) Mammalian tissue culture techniques (HeLa, HEK, CHO, macrophage, hybridnomas) SDS-PAGE, western blotting, & enzyme linked immunosorbent assay (ELISA) Empower, Mass Lynx, Laura, Astra software, some familiarity with Analyst UV-Vis spectrophotometry and some experience in gas chromatography (GC)