Chang Ki Yi

US Patent:

20210403885, Dec 30, 2021

Filed:

Sep 15, 2021

Appl. No.:

17/475810

Inventors:

- Bethesda MD, US
Chang Kyun Yi - Germantown MD, US
Katherine Marie McIntire - Sandy UT, US
Lawrence Elliot Samelson - Bethesda MD, US

Assignee:

The United States of America, as represented by the Secretary, Dept. of Health and Human Services - Bethesda MD

International Classification:

C12N 9/12
C07K 14/705
C07K 14/535
C07K 16/28
A61K 35/17
A61P 35/02
A61P 35/00

Abstract:

Chimeric polypeptides including (a) an extracellular targeting domain; (b) a transmembrane domain; (c) an intracellular linker for activation of T cells (LAT) domain or SLP-76 domain; and (d) an intracellular ZAP70 domain, wherein (a)-(d) are in N-terminal to C-terminal order are provided. Chimeric polypeptides including (a) an extracellular targeting domain; (b) a transmembrane domain; and (c) a ZAP70 domain, wherein (a)-(c) are in N-terminal to C-terminal order are also provided. In some embodiments, the chimeric polypeptide further includes a hinge domain, a signal sequence domain, and/or an intracellular signaling domain. Nucleic acid molecules encoding the chimeric polypeptides and expression vectors including the nucleic acids are also provided. Isolated cells (such as T cells or natural killer cells) expressing the chimeric polypeptides and methods of treating a subject with cancer with the isolated cells are provided.

US Patent:

20180356343, Dec 13, 2018

Filed:

Dec 2, 2016

Appl. No.:

15/780388

Inventors:

- Bethesda MD, US
Han Wen - Silver Spring MD, US
Jennifer Hong - Loredo TX, US
Chang Kuyn Yi - Germantown MD, US
Ambika Bumb - Greer SC, US
Susanta Kumar Sarkar - Rockville MD, US
Asit Kumar Manna - Rockville MD, US
Lawrence Elliot Samelson - Chevy Chase MD, US

International Classification:

G01N 21/64
G01N 33/533

Abstract:

Fiducial marker compositions comprising fluorescent nanodiamonds and methods of making and using the fiducial marker compositions are disclosed. The fiducial marker composition comprises a substrate, and a fluorescent nanodiamond immobilized on a surface of the substrate, wherein the substrate and immobilized fluorescent nanodiamond are optionally top coated with an inert top coating. The fiducial marker compositions are used in imaging methods to correct for drift and other alignment instabilities, and are particularly useful in super-resolution imaging.