Charles Raymond Gray

US Patent:

47849509, Nov 15, 1988

Filed:

Dec 16, 1986

Appl. No.:

6/810002

Inventors:

Frederick S. Hagen - Seattle WA
Mark J. Murray - Seattle WA
Sharon J. Busby - Seattle WA
Kathleen L. Berkner - Seattle WA
Margaret Y. Insley - Woodinville WA
Richard G. Woodbury - Seattle WA
Charles L. Gray - Seattle WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12N 1500
C12P 2102
C07H 1512
C07K 1300

US Classification:

435 68

Abstract:

Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of Factor VIIA. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as Factor VIIa.