Charles L Hershberger

US Patent:

6531308, Mar 11, 2003

Filed:

Oct 8, 1999

Appl. No.:

09/415277

Inventors:

Charles Lee Hershberger - Greenfield IN
Robert Allen Payson - Greenwood IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 1500

US Classification:

4352523, 4353201, 43525233, 435 691, 4352541, 4352542, 43525421, 536 2374, 536 237, 536 232

Abstract:

The invention provides cloned ketoreductase genes, vectors for expressing same, recombinant host cells that express said vector-borne genes, a method for stereospecifically reducing a ketone using a recombinant ketoreductase, or a recombinant host cell that expresses a cloned ketoreductase gene, as well as a method for generating polynucleotide sequences more conducive to recombinant expression.

US Patent:

20050009029, Jan 13, 2005

Filed:

Dec 3, 2002

Appl. No.:

10/495468

Inventors:

Christopher Frye - Plainfield IN, US
Charles Hershberger - Greenfield IN, US

International Classification:

C12Q001/68
C07H021/04
C12N001/20
C12N009/22
C12N015/86

US Classification:

435006000, 435069100, 435456000, 435252300, 435199000, 530399000, 536023200

Abstract:

The present invention provides an expression system for producing a target protein in a host cell comprising a homologously integrated gene encoding T7 RNA polymerase, and a nonintegrated gene encoding a target protein.

US Patent:

20060205041, Sep 14, 2006

Filed:

Dec 5, 2002

Appl. No.:

10/495479

Inventors:

Christopher Frye - Plainfield IN, US
Charles Hershberger - Greenfield IN, US

International Classification:

C12P 21/06
C07H 21/04
C12N 9/74

US Classification:

435100000, 435069100, 435214000, 435325000, 435320100, 536023200

Abstract:

Methods are disclosed for producing recombinant thrombin from precursor molecules that do not require activation by Factor Xa. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. Methods for purification of the thrombin precursors are also described. Thrombin precursors produced from transformed or transfected host cells are auto-activated or secreted in an activated form.

US Patent:

61365653, Oct 24, 2000

Filed:

Oct 18, 1999

Appl. No.:

9/269584

Inventors:

Elaine A. Best - Fort Collins CO
Charles Lee Hershberger - Greenfield IN
Christopher Carl Frye - Mooresville IN

Assignee:

Baxter Biotech Technology Sarl - Neuchatel
Eli Lilly and Company - Indianapolis IN

International Classification:

C12P 2104
C12P 2106
C07K 14805

US Classification:

435 696

Abstract:

The present invention relates to recombinant hemoglobin solutions containing reduced amounts of protoporphyrin IX and to methods of producing such hemoglobin solutions. The methods are accomplished by overexpressing the hemH gene in a suitable host cell or by increasing the production of hemH in a host cell, for example, by integration of the hemH gene into the chromosome of the host cell or by inserting multiple copies of the gene in host cells naturally having a hemH gene.

US Patent:

53957610, Mar 7, 1995

Filed:

Nov 27, 1991

Appl. No.:

7/801164

Inventors:

Charles L. Hershberger - New Palestine IN
Jeffrey L. Larson - Indianapolis IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 121
C12N 1570

US Classification:

43525233

Abstract:

Plasmid pHKY334, an expression vector for Met-Phe-Pro-Leu-(Asp). sub. 4 -Leu-BGH, and host cells containing plasmid pHKY334 are disclosed and claimed.

US Patent:

47328592, Mar 22, 1988

Filed:

Sep 5, 1984

Appl. No.:

6/647338

Inventors:

Charles L. Hershberger - New Palestine IN
Paul R. Rosteck - Beech Grove IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 914
C12N 120
C12N 100
C12N 1500
C12N 910
C12P 2100

US Classification:

435320

Abstract:

A novel method for protecting a bacterium from a naturally occurring bacteriophage and the cloning vectors and transformants for carrying out the aforementioned method are disclosed.

US Patent:

60080418, Dec 28, 1999

Filed:

May 2, 1997

Appl. No.:

8/850227

Inventors:

Christopher Carl Frye - Bloomington IN
Charles Lee Hershberger - Greenfield IN
Haichao Zhang - Lake Bluff IL

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 950
C12N 500
C12N 120
C07H 2104

US Classification:

435325

Abstract:

The present invention provides the cDNA sequence encoding bovine dipeptidylaminopeptidase 1. The invention demonstrates that bovine DAP 1 coded by a single cDNA. The mature protein is derived from a single polypeptide consisting of a signal peptide, and a major polypeptide which is processed to generate the a subunit, b subunit and g subunit.

US Patent:

45377154, Aug 27, 1985

Filed:

Oct 21, 1983

Appl. No.:

6/544338

Inventors:

LaVerne D. Boeck - Indianapolis IN
Gladys M. Clem - Indianapolis IN
Charles L. Hershberger - New Palestine IN
Marie T. Anderson - Indianapolis IN
Karl H. Michel - Indianapolis IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C07C10352
C12P 2104
A23K 122

US Classification:

2601125R

Abstract:

New glycopeptide antibiotic CUC/CSV which has the formula: ##STR1## wherein R is L-ristosaminyl; R. sub. 1 is the disaccharide mannosyl-glucosyl; and R. sub. 2 and R. sub. 3 are mannosyl, and its salts, particularly the pharmaceutically acceptable salts, are useful new antibiotics are active against gram-positive bacteria and increase feed-efficiency utilization and enhance milk production in ruminants.