Charles Karwowski Hoppel

US Patent:

20080199882, Aug 21, 2008

Filed:

Nov 5, 2007

Appl. No.:

11/935384

Inventors:

Dorr G. Dearborn - Mayfield Village OH, US
Iwona Yike - Chardon OH, US
Anne M. Distler - Westlake OH, US
Charles Hoppel - Cleveland Heights OH, US

Assignee:

CASE WESTERN RESERVE UNIVERSITY - Cleveland OH

International Classification:

G01N 33/53

US Classification:

435 71, 436501

Abstract:

A method of determining exposure of an individual to a macrocyclic trichothecene comprises isolating a sample of at least a portion of a naturally occurring protein from an individual, and detecting a reaction of the sample with a macrocyclic trichothecene. The macrocyclic trichothecene may be a product of , such as satratoxin G. The sample may be a blood protein or a metabolic product of a satratoxin adduct found in urine. The interaction of the protein and the macrocyclic trichothecene may be detected by any one or more of a number of methods. The sample may be a proteinase product of a naturally occurring protein. A kit for determining the exposure of an individual to a macrocyclic trichothecene comprises an antibody that reacts to a macrocyclic trichothecene covalently bound to an amino acid derived from a naturally occurring protein.