Cheng Chang David Lee

US Patent:

20030127329, Jul 10, 2003

Filed:

Jun 1, 2002

Appl. No.:

10/159914

Inventors:

Donald DeVoe - Bethesda MD, US
Cheng Lee - Ellicott City MD, US

International Classification:

G01N027/26
G01N027/447

US Classification:

204/454000, 204/451000, 204/601000

Abstract:

One embodiment of the invention relates to a microfluidic apparatus for controlling fluid flow velocity during electroosmotic flow. According to one aspect of the invention, a voltage applied to a gate electrode modulates flow velocity within an associated microchannel, where the gate voltage is separate from any voltage used to induce electroosmotic flow. According to another aspect of the invention, the flow control apparatus combines multiple gate electrodes to control flow in a microfluidic network. According to one embodiment of the invention, the flow control apparatus is fabricated in a planar silicon substrate. According to another embodiment of the invention, the flow control apparatus is fabricated using polymer materials.

US Patent:

20060019399, Jan 26, 2006

Filed:

Jul 1, 2005

Appl. No.:

11/171427

Inventors:

Brian Balgley - Annandale VA, US
Jonathan Cooper - Walkersville MD, US
Cheng Lee - Ellicott City MD, US
Donald DeVoe - Bethesda MD, US

International Classification:

G01N 33/00

US Classification:

436086000

Abstract:

Disclosed is a system for performing multiple analyses of protein and/or peptide samples and correlating the results of the analyses. The system comprises a sample inlet, a splitter means, at least two sample delivery capillaries, at least two sample deposition tools, and at least two sample collectors, wherein said splitter means is in fluid communication with the sample inlet and the sample delivery capillaries, and wherein liquid flow entering the splitter means is split into a number of sub-flows equal to the number of sample delivery capillaries. In one preferred embodiment, at least one microenzyme reactor is interfaced to a first sample delivery capillary in order to digest a protein sample within the capillary, while a second sample delivery capillary does not contain a microenzyme reactor, thereby enabling correlated analysis of the same protein sample in digested and undigested form. Methods for performing two or more analyses of protein and/or peptide samples and correlating the results of the analyses are also disclosed.

US Patent:

20060192107, Aug 31, 2006

Filed:

Oct 5, 2005

Appl. No.:

11/242842

Inventors:

Donald DeVoe - Bethesda MD, US
Yingxin Wang - Columbia MD, US
Cheng Lee - Ellicott City MD, US
Yan Li - Bethesda MD, US

International Classification:

B01D 59/44

US Classification:

250288000

Abstract:

Disclosed are an apparatus, system, and method for performing electrospray of biomolecules, particularly peptides, polypeptides, and proteins. The apparatus comprises at least (1) a microfluidic substrate for containing an electrospray microchannel for delivering analyte molecules to a side edge of the substrate, and (2) a porous membrane attached to the side edge for performing electrospray from the exposed membrane surface. In one preferred embodiment, the exposed membrane surface is positioned above a target surface for depositing analyte molecules onto the target surface by electrospray. In another preferred embodiment, a proteolytic enzyme is bound to the porous membrane for performing protein digestion during electrospray.

US Patent:

20070213295, Sep 13, 2007

Filed:

Jan 16, 2007

Appl. No.:

11/623625

Inventors:

Cheng Lee - Houston TX, US
Jianfa Zhang - Houston TX, US

International Classification:

A61K 31/7076

US Classification:

514047000

Abstract:

The present invention relates to the discovery the 5′-AMP and analogues thereof can be used to induce a state of torpor or suspended animation in subjects, as exemplified by studies carried out in laboratory mice. In these studies, mice were injected with high doses of 5′-AMP, which was found to result in a decoupling of the animals' body temperature regulation mechanism accompanied by a reduction in the animals' core body temperature, which tended to lower towards the ambient environmental temperature. It was further discovered that the introduction of high levels of 5′-AMP resulted in an induction of fat regulation genes such as procolipase (Clps) in tissues and organs that do not normally express Clps, this in turn was accompanied by a shift in metabolism from a primarily glycolytic energy metabolism (which is inhibited at lower temperatures) to one that relied primarily on the liberation and metabolism of free fatty acids. Substantial medical and other applications that arise out of this discovery are also disclosed.

US Patent:

20110059916, Mar 10, 2011

Filed:

May 17, 2010

Appl. No.:

12/781760

Inventors:

Cheng Chi Lee - Houston TX, US
Jianfa Zhang - Houston TX, US

International Classification:

A61K 31/7076
A61P 3/00
A61P 9/00
A61P 11/06
A61P 25/00
A61P 25/28
A61P 35/00

US Classification:

514 47

Abstract:

The present invention relates to the discovery the 5′-AMP and analogues thereof can be used to induce a state of torpor or suspended animation in subjects, as exemplified by studies carried out in laboratory mice. In these studies; mice were injected with high doses of 5′-AMP, which was found to result in a decoupling of the animals' body temperature regulation mechanism accompanied by a reduction in the animals' core body temperature, which tended to lower towards the ambient environmental temperature. It was further discovered that the introduction of high levels of 5′-AMP resulted in an induction of fat regulation genes such as procolipase (Clps) in tissues and organs that do not normally express Clps, this in turn was accompanied by a shift in metabolism from a primarily glycolytic energy metabolism (which is inhibited at lower temperatures) to one that relied primarily on the liberation and metabolism of free fatty acids. Substantial medical and other applications that arise out of this discovery are also disclosed.

US Patent:

43225012, Mar 30, 1982

Filed:

Sep 22, 1980

Appl. No.:

6/189414

Inventors:

Cheng H. Lee - Wyckoff NJ
Jay M. S. Henis - Creve Coeur MO

Assignee:

Monsanto Company - St. Louis MO

International Classification:

B01J 4112
C08F 832

US Classification:

521 32

Abstract:

A process is disclosed for quaternizing reactive substituent haloalkyl groups in hollow fiber polymer membranes wherein such haloalkyl groups are reacted with an amine quaternizing agent in the presence of an electrolyte compound and, preferably, in the presence of a cationic quaternary ammonium halide surfactant. In addition, there is disclosed a process of quaternizing the reactive haloalkyl groups in anisotropic hollow fiber polymer membranes by contacting and permeating a solution of an amine quaternizing agent from the surface of the membrane which is to be exposed to a dialysis feed solution.

US Patent:

53345371, Aug 2, 1994

Filed:

Nov 14, 1991

Appl. No.:

7/791309

Inventors:

Cheng S. Lee - Columbia MD
Ping Y. Huang - Baltimore MD

Assignee:

The University of Maryland - College Park MD

International Classification:

G01N 33543
G01N 33536

US Classification:

436518

Abstract:

The complexing of an antibody-antigen binding pair is determined by observing the change in fluorescence of a pH-sensitive fluorochrome attached to one of the members of the binding pair. When the binding is conducted in a solution having a pH other than the isoelectric point of the antibody, there will be a change in the pH of the microenviromnent surrounding the fluorochrome. This change will correspond to a change in the observed fluorescent intensity. Either member of the binding pair can be labeled, and combined with that member whose presence is suspect, in an immunoassay.

US Patent:

20140342949, Nov 20, 2014

Filed:

Mar 14, 2014

Appl. No.:

14/213015

Inventors:

YuHuang WANG - Laurel MD, US
Jia HUANG - Shanghai, CN
Allen NG - Silver Spring MD, US
Yanmei PIAO - College Park MD, US
Cheng S. LEE - Ellicott City MD, US

Assignee:

University of Maryland, College Park - College Park MD

International Classification:

G01N 27/22
B01J 19/12
G01N 33/00
G01N 33/543

US Classification:

506 32, 422 8202, 422 69, 506 39

Abstract:

The present invention is directed to tube-in-a-tube electronic materials and electronic chemical sensors comprising tube-in-a-tube configurations such as covalently functionalized double-walled carbon nanotubes.