Chris Bock

US Patent:

7964356, Jun 21, 2011

Filed:

Jul 9, 2009

Appl. No.:

12/499967

Inventors:

Dominic Zichi - Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Longmont CO, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

Assignee:

SomaLogic, Inc. - Boulder CO

International Classification:

C12Q 1/68
C07H 21/02

US Classification:

435 6, 536 231

Abstract:

The present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. This invention relates to the field of diagnostic histology, cytology, histopathology, and cytopathology methods and reagents for the detection of various disease states. More specifically, the invention relates to the use of aptamers in histologic, cytologic, histopathic, and/or cytopathic diagnostic methods. Aptamers may be provided that react with specific target molecules contained within a histological or cytological sample. Aptamers may be used to assess localization, relative density, and presence or absence of one or more target. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected.

US Patent:

8404830, Mar 26, 2013

Filed:

Dec 15, 2010

Appl. No.:

12/969239

Inventors:

Dominic Zichi - Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Denver CO, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

Assignee:

SomaLogic, Inc. - Boulder CO

International Classification:

C07H 19/00
C07H 21/04

US Classification:

536 245, 536 2854

Abstract:

The present disclosure is directed to the field of nucleic acid chemistry, specifically to 5-position modified uridines, as well as, oligonucleotides comprising said 5-position modified uridines. The present disclosure describes methods for producing aptamers comprising said 5-position modified uridines that are capable of binding to target molecules.

US Patent:

20090004667, Jan 1, 2009

Filed:

Jul 17, 2008

Appl. No.:

12/175434

Inventors:

Dominic Zichi - Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Longmont CO, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

Assignee:

SOMALOGIC, INC. - Boulder CO

International Classification:

C12Q 1/68
C12Q 1/70
C07H 21/00

US Classification:

435 6, 536 231, 435 5

Abstract:

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

US Patent:

20110245479, Oct 6, 2011

Filed:

May 23, 2011

Appl. No.:

13/113261

Inventors:

Dominic Zichi - Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Denver CO, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

Assignee:

SOMALOGIC, INC. - Boulder CO

International Classification:

C07H 21/04
C40B 10/00

US Classification:

536 231, 506 1

Abstract:

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

US Patent:

20120115752, May 10, 2012

Filed:

Jul 9, 2010

Appl. No.:

13/382493

Inventors:

Dominic Zichi - Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Denver CO, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US
Thale C. Jarvis - Boulder CO, US
Jeffrey D. Carter - Longmont CO, US

Assignee:

SOMALOGIC, INC. - Boulder CO

International Classification:

C40B 30/04
C12Q 1/68
C12Q 1/70
C07H 21/00
C12Q 1/02
B82Y 15/00

US Classification:

506 9, 536 231, 435 29, 435 61, 435 5, 977774, 977902

Abstract:

The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.

US Patent:

20190330634, Oct 31, 2019

Filed:

May 16, 2019

Appl. No.:

16/414026

Inventors:

- Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Cleveland Heights OH, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

International Classification:

C12N 15/115
C12Q 1/6816
C12Q 1/6832
C12Q 1/6834
G01N 33/53
G01N 33/58
C12N 9/74
C12Q 1/6811
C12N 15/10

Abstract:

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

US Patent:

20190264208, Aug 29, 2019

Filed:

May 16, 2019

Appl. No.:

16/413989

Inventors:

- Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Cleveland Heights OH, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

International Classification:

C12N 15/115
C12Q 1/6816
C12Q 1/6832
C12Q 1/6834
G01N 33/53
G01N 33/58
C12N 9/74
C12Q 1/6811
C12N 15/10

Abstract:

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

US Patent:

20180155725, Jun 7, 2018

Filed:

Jan 23, 2018

Appl. No.:

15/878161

Inventors:

- Boulder CO, US
Sheri K. Wilcox - Longmont CO, US
Chris Bock - Cleveland Heights OH, US
Daniel J. Schneider - Arvada CO, US
Bruce Eaton - Longmont CO, US
Larry Gold - Boulder CO, US

Assignee:

SomaLogic, Inc. - Boulder CO

International Classification:

C12N 15/115
C12N 15/10
G01N 33/53

Abstract:

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.