Chris Lyon

US Patent:

20130203128, Aug 8, 2013

Filed:

Oct 6, 2011

Appl. No.:

13/824315

Inventors:

Justin T. Stege - San Diego CA, US
Alexander Varvak - Netanya IL, US
John Poland - San Diego CA, US
Chris S. Lyon - San Diego CA, US
Shaun Healey - Carlsbad CA, US
Peter Luginbuhl - San Diego CA, US

Assignee:

BP Corporation North America Inc, - Houston TX

International Classification:

C12N 9/42
C12P 19/02

US Classification:

435105, 435201, 435162, 536 232, 4353201, 43525411, 4352543, 4352545, 4352546, 4352547, 4352549, 4352542, 43525421, 43525422, 43525423, 4352523

Abstract:

In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

US Patent:

20190010419, Jan 10, 2019

Filed:

Sep 13, 2018

Appl. No.:

16/130575

Inventors:

- TE Heerlen, NL
William BARCLAY - Boulder CO, US
Micah BLAZER - San Diego CA, US
Jacob BORDEN - Lake Charles LA, US
Adam Burja - Columbia MD, US
Daniel DONG - Woodstock MD, US
Armando DURAZO - San Diego CA, US
Jean-Charles DUMENIL - Little Chaufront, GB
Arthur EDGE - Naperville IL, US
Jon HANSEN - Chandler AZ, US
Alexandra HOFLER - San Diego CA, US
David JEFFERS - Woking Surray Guziable, GB
Chris LYON - San Diego CA, US
Vidya PAI - Rockville MD, US
Martin J. SELLERS - Workingham, GB
Ginger SHANK - Winchester KY, US
Justin STEGE - San Diego CA, US

International Classification:

C11B 1/02
C10L 1/02
C12N 9/26
C12N 1/06
C12N 9/24
C11B 1/12
C11B 1/10
C12P 7/64

Abstract:

Methods and systems used to extract lipids suitable in production of biofuels from a fermentation broth may include using heat to pre-treat the fermentation broth in order to more easily extract a product from oleaginous microorganisms in the broth. Additionally or alternatively, a combination of enzymes including amylase, 1-4 mannosidase, and 1-3 mannosidase may be used to break down cell walls of the oleaginous microorganisms. Residual broth water may be recycled and used as imbibition water for washing a process feedstock to extract sugar.

US Patent:

20160355749, Dec 8, 2016

Filed:

Dec 18, 2014

Appl. No.:

15/106752

Inventors:

Kirk APT - Columbia MD, US
William BARCLAY - Boulder CO, US
Micah BLAZER - Columbia MD, US
Jacob BORDEN - Lake Charles LA, US
Adam BURJA - Halifax, CA
Daniel Dong - Woodstock MD, US
Armando DURAZO - Columbia MD, US
Jean-Charles DUMENIL - Columbia, GB
Arthur EDGE - Columbia MD, US
Jon HANSEN - Chandler AZ, US
Alexandra HOFLER - Columbia MD, US
David JEFFERS - Columbia, GB
Chris LYON - San Diego CA, US
Vidya PAI - Rockville MD, US
Martin J. SELLERS - Wokingham-Berkshire, GB
Ginger SHANK - Winchester KY, US
Justin STEGE - Columbia MD, US

Assignee:

DSM IP ASSETS B.V. - TE Heerlen

International Classification:

C11B 1/02
C12N 9/26
C12P 7/64
C11B 1/10
C12N 1/06
C10L 1/02
C12N 9/24

Abstract:

Methods and systems used to extract lipids suitable in production of biofuels from a fermentation broth may include using heat to pre-treat the fermentation broth in order to more easily extract a product from oleaginous microorganisms in the broth. Additionally or alternatively, a combination of enzymes including amylase, 1-4 mannosidase, and 1-3 mannosidase may be used to break down cell walls of the oleaginous microorganisms. Residual broth water may be recycled and used as imbibition water for washing a process feedstock to extract sugar.

US Patent:

20150329880, Nov 19, 2015

Filed:

Aug 3, 2015

Appl. No.:

14/816992

Inventors:

- Houston TX, US
Alexander Varvak - Netanya, IL
John Poland - San Diego CA, US
Chris S. Lyon - San Diego CA, US
Shaun Healey - San Diego CA, US
Peter Luginbuhl - San Diego CA, US

International Classification:

C12P 7/10
C12N 9/42

Abstract:

The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.

US Patent:

20140147894, May 29, 2014

Filed:

Oct 6, 2011

Appl. No.:

13/824317

Inventors:

Justin T. Stege - San Diego CA, US
Alexander Varvak - Netanya, IL
John Poland - San Diego CA, US
Chris S. Lyon - San Diego CA, US
Shaun Healey - San Diego CA, US
Peter Luginhuhl - San Diego CA, US

Assignee:

BP Corporation North America Inc. - Houston TX

International Classification:

C12N 9/42
C12P 7/14
C12P 19/02
C12P 19/14

US Classification:

435 99, 435162, 435188, 435209, 4352523, 43525411, 4352542, 43525421, 43525422, 43525423, 4352543, 4352545, 4352546, 4352549, 4353201, 536 232, 536 253

Abstract:

The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.