Christopher A Armstrong

US Patent:

7452664, Nov 18, 2008

Filed:

Dec 15, 1999

Appl. No.:

09/461580

Inventors:

Leonard Guarente - Chestnut Hill MA, US
Shin-ichiro Imai - Somerville MA, US
Christopher Armstrong - Cambridge MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435 6, 435 4, 435 5, 435 76, 435 15

Abstract:

Methods of identifying agents which alter the NAD-dependent acetylation status and mono-ADP-ribosylation of nuclear proteins are disclosed. The methods further include identifying agents which alter the life span or aging of a cell or an organism by determining the level of NAD-dependent acetylation and/or ADP ribosylation of a nuclear protein. The invention also relates to a mammalian Sir2 protein which acetylates or deacetylates nuclear proteins in a NAD-dependent manner and has mono-ADP-ribosyltransferase activity. Host cells producing the Sir2 protein and antibodies to the Sir2 protein are also provided.

US Patent:

8642284, Feb 4, 2014

Filed:

Dec 13, 2000

Appl. No.:

09/735786

Inventors:

Leonard Guarente - Chestnut Hill MA, US
Shin-Ichiro Imai - St. Louis MO, US
Christopher Armstrong - Jamaica Plain MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12Q 1/02
C12N 9/00

US Classification:

435 29, 435183

Abstract:

Methods of identifying agents which alter the NAD-dependent deacetylation activity of a Sir2 protein or a fragment of a Sir2 protein are disclosed. The acetylated protein can be a nuclear protein, such as a histone protein, or a cytoplasmic protein. The Sir2 protein employed in the methods can include at least a core domain of a Sir2 protein, such as a human Sir2 protein.

US Patent:

20030032750, Feb 13, 2003

Filed:

Aug 2, 2001

Appl. No.:

09/921322

Inventors:

Keunsuk Chang - North Kingstown RI, US
Michael Narkevicius - Richmond RI, US
Hiroyuki Kato - East Greenwich RI, US
Christopher Armstrong - North Kingstown RI, US
Mario Scarati - Milan, IT
Enrico Ballico - Marino, IT
Angelo del Lupo - Vasto, IT

Assignee:

Toray Plastics (America), Inc.

International Classification:

C08F110/00

US Classification:

526/348100, 428/461000, 427/460000, 264/288400, 428/518000, 428/515000

Abstract:

A laminate film comprising a polyolefin polymer layer having a surface treated by a flame or corona discharge treatment method and biaxially oriented at about 5.5-10.0 stretch ratio in the machine direction and about 7.0-12.0 stretch ratio in the transverse direction which imparts superior machine direction tensile properties such as Young's modulus of about 350,000 to about 400,000 psi or greater, elongation of 120% or less, and tensile strength of about 27,000 to about 30,000 psi or greater; and a metal layer having an optical density of at least about 1.8 deposited on said treated surface of said polyolefin-based resin layer.

US Patent:

20090155834, Jun 18, 2009

Filed:

Sep 12, 2008

Appl. No.:

12/209847

Inventors:

LEONARD GUARENTE - CHESTNUT HILL MA, US
SHIN-ICHIRO IMAI - SOMERVILLE MA, US
CHRISTOPHER ARMSTRONG - CAMBRIDGE MA, US

Assignee:

MASSACHUSETTS INSTITUTE OF TECHNOLOGY - CAMBRIDGE MA

International Classification:

C12Q 1/02
C12N 9/00

US Classification:

435 29, 435183

Abstract:

Methods of identifying agents which alter the NAD-dependent acetylation status and mono-ADP-ribosylation of nuclear proteins are disclosed. The methods further include identifying agents which alter the life span or aging of a cell or an organism by determining the level of NAD-dependent acetylation and/or ADP ribosylation of a nuclear protein. The invention also relates to a mammalian Sir2 protein which acetylates or deacetylates nuclear proteins in a NAD-dependent manner and has mono-ADP-ribosyltransferase activity. Host cells producing the Sir2 protein and antibodies to the Sir2 protein are also provided.