Christopher F Barnett

US Patent:

6455288, Sep 24, 2002

Filed:

Apr 8, 1993

Appl. No.:

08/044857

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Dade Behring Marburg GmbH - Marburg
Genencor International, Inc. - San Francisco CA

International Classification:

C12N 1552

US Classification:

435190, 4352523, 4353201, 536 232

Abstract:

The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

US Patent:

20100138337, Jun 3, 2010

Filed:

Dec 1, 2008

Appl. No.:

12/325403

Inventors:

Christopher R. Barnett - San Francisco CA, US
Elwin Kongeang Sam - Tustin CA, US
Geethika Uriti - Fremont CA, US

Assignee:

FOX DIGITAL ENTERPRISES, INC. - Los Angeles CA

International Classification:

G06Q 40/00

US Classification:

705 39

Abstract:

In one example, a media purchase system is provided. The media purchase system includes: a media manager module that selectively coordinates a media file with an operating scenario in a video game; a selection manager module that manages a selection of the media file for purchase in the video game; and a media purchase module that manages a purchase of the selection in the video game.

US Patent:

61034649, Aug 15, 2000

Filed:

Jun 5, 1995

Appl. No.:

8/463461

Inventors:

Timothy Fowler - Belmont CA
Christopher C. Barnett - South San Francisco CA
Sharon Shoemaker - Fairfield CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12Q 168
C07H 2104

US Classification:

435 6

Abstract:

A process for expressing extracellular. beta. -glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered. beta. -glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of. beta. -glucosidase is also disclosed.

US Patent:

62970372, Oct 2, 2001

Filed:

Feb 10, 1994

Appl. No.:

8/194664

Inventors:

Christopher C. Barnett - South San Francisco CA
Colin Mitchinson - Half Moon Bay CA
Scott D. Power - San Bruno CA
Carol A. Requadt - Tiburon CA

International Classification:

C12N 928
C12N 1556

US Classification:

435202

Abstract:

Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

US Patent:

60227257, Feb 8, 2000

Filed:

Jun 5, 1995

Appl. No.:

8/462090

Inventors:

Timothy Fowler - Belmont CA
Christopher C. Barnett - South San Francisco CA
Sharon Shoemaker - Fairfield CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 1555
C12N 1580
C12Q 168

US Classification:

435209

Abstract:

A process for expressing extracellular. beta. -glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered. beta. -glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of. beta. -glucosidase is also disclosed.

US Patent:

58245324, Oct 20, 1998

Filed:

Jun 6, 1995

Appl. No.:

8/468220

Inventors:

Christopher C. Barnett - South San Francisco CA
Colin Mitchinson - Half Moon Bay CA
Scott D. Power - San Bruno CA
Carol A. Requadt - Tiburon CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 1556
C12N 928
C12N 121
C12N 1563

US Classification:

435202

Abstract:

Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

US Patent:

60338903, Mar 7, 2000

Filed:

May 22, 1995

Appl. No.:

8/445463

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Behring Diagnostics GmbH - Marburg

International Classification:

C12N 904

US Classification:

435190

Abstract:

The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD. sup. + dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

US Patent:

60905670, Jul 18, 2000

Filed:

May 22, 1995

Appl. No.:

8/445464

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Behringwerke AG - Marburg

International Classification:

G01N 3353
G01N 33542

US Classification:

435 79

Abstract:

Methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD. sup. + dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.