Christopher S Chen

US Patent:

7288394, Oct 30, 2007

Filed:

Sep 23, 2003

Appl. No.:

10/668679

Inventors:

Emanuele Ostuni - Watertown MA, US
Christopher S. Chen - Baltimore MD, US
Donald E. Ingber - Boston MA, US
George M. Whitesides - Newton MA, US

Assignee:

President & Fellows of Harvard College - Cambridge MA
Children's Medical Center Corporation - Boston MA

International Classification:

C12N 11/02
C12N 11/08
C07K 17/02
C07K 17/08

US Classification:

435177, 435180, 530812, 530815

Abstract:

A method of patterning materials, such as proteins, on a contoured surface by depositing them onto protrusions on a surface, and a cell containment device that may be constructed by this method, are provided. The method may involve selectively depositing a material on a substrate including a contoured surface including protrusions and recesses. By applying a first fluid to the contoured surface and allowing the first fluid to distribute across only a portion of the contoured surface, a material may be deposited on the protrusions and not the recesses, or on the recesses and not the protrusions. Such a method may be used to selectively pattern cells or other materials on substrates.

US Patent:

20080145908, Jun 19, 2008

Filed:

Sep 14, 2007

Appl. No.:

11/901277

Inventors:

Emanuele Ostuni - Watertown MA, US
Christopher S. Chen - Baltimore MD, US
Donald E. Ingber - Boston MA, US
George M. Whitesides - Newton MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12N 11/08

US Classification:

435180

Abstract:

The present invention is directed to a method of patterning materials, such as proteins, on a contoured surface by depositing them onto protrusions on the surface and to a cell containment device that may be constructed by this method. In one embodiment, the present invention is directed to a method of selectively depositing a material on a substrate including a contoured surface including a protrusion and a recess. The method includes applying a first fluid to the contoured surface of the substrate and allowing the first fluid to distribute across a portion of the contoured surface such that the first fluid contacts the protrusion and not the recess. The method also includes allowing a first material to deposit on the substrate where the substrate is in contact with the first fluid. Optionally, this method may further include applying a second fluid to the contoured surface of the substrate, allowing the second fluid to distribute across a portion of the contoured surface such that the second fluid contacts the recess, and allowing a second material to deposit on the substrate where the substrate is in contact with the second fluid. Optionally, the method may still further include applying a third fluid to the contoured surface of the substrate, allowing the third fluid to distribute across a portion of the contoured surface, and allowing a third material with an affinity for one of the first material and the second material to deposit on the substrate only where the one of the first material and the second material is deposited. In one embodiment of this method the first material is a cytophobic material, the second material is a cytophilic material and the third material is a cell.In another embodiment, the present invention is directed to a cell containment device, including a substrate having a contoured surface, a recess in the surface of the substrate, a cytophobic material connected to the surface of the substrate outside the recess and a cytophilic material connected to the recess.

US Patent:

20220354994, Nov 10, 2022

Filed:

Apr 22, 2022

Appl. No.:

17/727293

Inventors:

- Cambridge MA, US
- Boston MA, US
Christopher S. Chen - Newton MA, US

International Classification:

A61L 27/52
A61L 27/38

Abstract:

Engineered human tissue seed constructs are provided that are suitable for implantation in subjects. Methods of making and using the engineered tissue seed constructs are provided.

US Patent:

20210213173, Jul 15, 2021

Filed:

Mar 26, 2021

Appl. No.:

17/213436

Inventors:

- Cambridge MA, US
- Boston MA, US
Christopher S. CHEN - Newton MA, US

International Classification:

A61L 27/52
A61L 27/38

Abstract:

Engineered human tissue seed constructs are provided that are suitable for implantation in subjects. Methods of making and using the engineered tissue seed constructs are provided.

US Patent:

20210115369, Apr 22, 2021

Filed:

Dec 23, 2020

Appl. No.:

17/132482

Inventors:

- Boston MA, US
Anant Chopra - Waltham MA, US
Alice Elizabeth White - Brookline MA, US
Christopher S. Chen - Newton MA, US

International Classification:

C12M 3/06
A61M 5/168
G09B 23/30
C12M 1/26
F16K 99/00
C12N 5/077

Abstract:

A valve includes a body including an inner bore extending between a first port and a second port, a seat, and one or more restrainers and a disk that is moveable between the seat and the one or more restrainers such that a first pressure that is less than 1 pascal and applied in a first direction causes the disk to move from a first position towards a second position to permit fluid communication between the first port and the second port. A metamaterial scaffold including a structure defining a lumen, at least a portion of an outer or non-lumen surface of the structure is coated with a plurality of biological cells, and wherein the structure is composed of a metamaterial.

US Patent:

20200017813, Jan 16, 2020

Filed:

Jul 10, 2019

Appl. No.:

16/508033

Inventors:

- Boston MA, US
Anant Chopra - Waltham MA, US
Alice Elizabeth White - Brookline MA, US
Christopher S. Chen - Newton MA, US

International Classification:

C12M 3/06
A61M 5/168
G09B 23/30
C12N 5/077
C12M 1/26
F16K 99/00

Abstract:

A valve includes a body including an inner bore extending between a first port and a second port, a seat, and one or more restrainers and a disk that is moveable between the seat and the one or more restrainers such that a first pressure that is less than 1 pascal and applied in a first direction causes the disk to move from a first position towards a second position to permit fluid communication between the first port and the second port. A metamaterial scaffold including a structure defining a lumen, at least a portion of an outer or non-lumen surface of the structure is coated with a plurality of biological cells, and wherein the structure is composed of a metamaterial.

US Patent:

20190076578, Mar 14, 2019

Filed:

Oct 7, 2016

Appl. No.:

15/765526

Inventors:

- Cambridge MA, US
- Boston MA, US
Christopher S. CHEN - Newton MA, US

International Classification:

A61L 27/52
A61L 27/38

Abstract:

Engineered human tissue seed constructs are provided that are suitable for implantation in subjects. Methods of making and using the engineered tissue seed constructs are provided.