Christopher E Dillon

US Patent:

20050251872, Nov 10, 2005

Filed:

Sep 5, 2003

Appl. No.:

10/655872

Inventors:

James Bear - Durham NC, US
Christopher Dillon - Cambridge MA, US
Douglas Rubinson - Cambridge MA, US
Luk Van Parijs - Falmouth MA, US

International Classification:

A01K067/00
C12N007/00
C12N015/867

US Classification:

800008000, 435456000, 435235100

Abstract:

The present invention provides new lentiviral vectors, including lentiviral transfer plasmids and infectious lentiviral particles. Lentiviral vectors of the invention were designed to offer a number of desirable features including reduced size, convenient cloning sites (including multiple cloning sites and sites for particularly useful restriction enzymes), loxP sites, self-inactivating LTRs, etc. Certain of the vectors are optimized for expression of reporter genes and/or for expression of siRNAs or shRNAs within eukaryotic cells. The invention also provides three and four plasmid lentiviral expression systems. In addition, the invention provides a variety of methods for using the vectors including gene silencing in cells and transgenic animals, and methods of treating disease.