Christopher J Ellison

US Patent:

20210380971, Dec 9, 2021

Filed:

Oct 3, 2019

Appl. No.:

17/282694

Inventors:

- San Diego CA, US
Damek Spacek - Palo Alto CA, US
Christopher E. Ellison - San Francisco CA, US
Samuel Levy - San Francisco CA, US

Assignee:

Bluestar Genomics, Inc. - San Diego CA

International Classification:

C12N 15/10
C12Q 1/6806

Abstract:

The invention provides a method for the analysis of a biological sample to determine multiple types of information therefrom in a streamlined, combined workflow, where all information is obtained in a sequencing-based analysis. The information includes the presence and concentration of specific plasma proteins in a blood sample; the number, location, and types of histone modifications associated with cell-free DNA obtained from the same sample; the sequence of cfRNA and cfDNA in the cell-free DNA sample; and epigenetic information pertaining to the cell-free DNA, such as hydroxymethylation and methylation profiles, i.e., the distribution of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) residues, respectively. The invention additionally pertains to a classical sequencing-based method for analyzing a biological sample to determine one or more non-classical sequence features of the sample. Compositions, kits, and related methods are also provided, including an embodiment in which truncated sequencing adapters are used in conjunction with barcoded PCR primers.